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    - PDB-2ix8: MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME -

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    Basic information

    Entry
    Database: PDB / ID: 2ix8
    TitleMODEL FOR EEF3 BOUND TO AN 80S RIBOSOME
    DescriptorELONGATION FACTOR 3A
    KeywordsNUCLEOTIDE-BINDING / PROTEIN BIOSYNTHESIS / PHOSPHORYLATION / ELONGATION FACTOR / RNA-BINDING / ATP-BINDING / RRNA-BINDING
    Specimen sourceSaccharomyces cerevisiae / yeast / BAKER'S YEAST /
    MethodElectron microscopy (6 A resolution / Single particle / Vitreous ice (cryo EM))
    AuthorsAndersen, C.B.F. / Becker, T. / Blau, M. / Anand, M. / Halic, M. / Balar, B. / Mielke, T. / Boesen, T. / Pedersen, J.S. / Spahn, C.M.T. / Kinzy, T.G. / Andersen, G.R. / Beckmann, R.
    CitationNature, 2006, 443, 663-668

    Nature, 2006, 443, 663-668 StrPapers
    Structure of eEF3 and the mechanism of transfer RNA release from the E-site.
    Christian B F Andersen / Thomas Becker / Michael Blau / Monika Anand / Mario Halic / Bharvi Balar / Thorsten Mielke / Thomas Boesen / Jan Skov Pedersen / Christian M T Spahn / Terri Goss Kinzy / Gregers R Andersen / Roland Beckmann

    DateDeposition: Jul 7, 2006 / Release: Jul 10, 2007 / Last modification: Apr 14, 2010

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    Assembly

    Deposited unit
    A: ELONGATION FACTOR 3A

    109 kDa, 1 molecules
    Theoretical massNumber of molelcules
    Total
    (without water)
    109,4421
    Polyers109,4421
    Non-polymers00
    Water0

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    #1idetical with deposited unit / defined by author&software (PQS) / Symmetry operations: (identity)x1
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    Components

    #1polypeptide(L) / ELONGATION FACTOR 3A / TRANSLATION ELONGATION IN FUNGI ABC-TYPE ATPASE TRNA RELEASE / EF-3A, EF-3, TRANSLATION ELONGATION FACTOR 3A, EUKARYOTIC ELONGATION FACTOR 3, EEF3, YEAST ELONGATION FACTOR 3 / Fragment: RESIDUES 1-976 / Source: SACCHAROMYCES CEREVISIAE (gene. exp.) / References: UniProt: P16521

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    Experimental details

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    Experiment

    ExperimentMethod: ELECTRON MICROSCOPY
    EM experimentReconstruction method: SINGLE PARTICLE / Specimen type: VITREOUS ICE (CRYO EM)

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    Sample preparation

    Assembly of specimenName: 80S-RNC-EEF3-AMP-PNP COMPLEX / Aggregation state: PARTICLE
    Buffer solutionName: 20MM HEPES,PH 7.5,10MM MG(OAC)2,150MM KOAC 1MM DTT,0.05% NIKKOL, 125MM SUCROSE,0.01MG -ML-1 CYCLOHEXIMIDE,0.5MM AMP-PNP,0.1MM NEOMYCIN, 0.3% DESOXYBIGCHAPS
    Sample preparationpH: 7.5 / Sample conc.: 0.17 mg/ml
    Specimen supportDetails: HOLEY CARBON
    VitrificationDetails: LIQUID ETHANE

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    Electron microscopy imaging

    MicroscopyMicroscope model: FEI TECNAI F30 / Date: Feb 11, 2005
    Electron gunAccelerating voltage: 300 kV / Illumination mode: LOW DOSE
    Electron lensMode: BRIGHT FIELD / Nominal magnification: 39000 X / Calibrated magnification: 38900 X / Nominal defocus max: 3500 nm / Nominal defocus min: 1200 nm / Cs: 2.7 mm
    Specimen holderTemperature: 95 K / Tilt angle min: 0 deg.
    CameraType: KODAK SO-163 FILM
    EM image scansNumber digital images: 141
    Radiation wavelengthRelative weight: 1

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    Processing

    Image selectionSoftware name: SPIDER
    EM single particle entitySymmetry type: ASYMMETRIC
    Least-squares processHighest resolution: 6 A
    Refine hist #LASTHighest resolution: 6 A
    Number of atoms included #LASTProtein: 7599 / Nucleic acid: 0 / Ligand: 0 / Solvent: 0 / Total: 7599

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