+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-22397 | |||||||||
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タイトル | T module | |||||||||
マップデータ | Tail module of Mediator complex | |||||||||
試料 |
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キーワード | Transcription (転写 (生物学)) / Activation (活性化) / PolII | |||||||||
機能・相同性 | 機能・相同性情報 core mediator complex / mediator complex / transcription coregulator activity / regulation of transcription by RNA polymerase II 類似検索 - 分子機能 | |||||||||
生物種 | Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) / Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.58 Å | |||||||||
データ登録者 | Zhang HQ / Chen DC / Bushnell DA / Kornberg RD | |||||||||
引用 | ジャーナル: Mol Cell / 年: 2021 タイトル: Mediator structure and conformation change. 著者: Heqiao Zhang / Dong-Hua Chen / Rayees U H Mattoo / David A Bushnell / Yannan Wang / Chao Yuan / Lin Wang / Chunnian Wang / Ralph E Davis / Yan Nie / Roger D Kornberg / 要旨: Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription ...Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription machinery. Previous structural studies revealed a relatively small part of Mediator and none of the gene activator-binding regions. We have determined the cryo-EM structure of the Mediator at near-atomic resolution. The structure reveals almost all amino acid residues in ordered regions, including the major targets of activator proteins, the Tail module, and the Med1 subunit of the Middle module. Comparison of Mediator structures with and without pol II reveals conformational changes that propagate across the entire Mediator, from Head to Tail, coupling activator- and pol II-interacting regions. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_22397.map.gz | 398.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-22397-v30.xml emd-22397.xml | 18.6 KB 18.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_22397_fsc.xml | 18.2 KB | 表示 | FSCデータファイル |
画像 | emd_22397.png | 130 KB | ||
Filedesc metadata | emd-22397.cif.gz | 8.1 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-22397 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-22397 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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-マップ
ファイル | ダウンロード / ファイル: emd_22397.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Tail module of Mediator complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.096 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : T module complex
全体 | 名称: T module complex |
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要素 |
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-超分子 #1: T module complex
超分子 | 名称: T module complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
-分子 #1: Mediator of RNA polymerase II transcription subunit 5
分子 | 名称: Mediator of RNA polymerase II transcription subunit 5 タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類) 株: DSM 1495 / CBS 144.50 / IMI 039719 |
分子量 | 理論値: 118.784406 KDa |
組換発現 | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
配列 | 文字列: MVTVTDPLTA RLEAAIKAWS DFFSDAEHER LDPAIFADQS QTLFANHPLA PVPLADLLLR PTPSNRECVD QRTLQYLQVL QKQGRITTA AVLRALYKYS TAHTRAQTPD GKPKHGAGDS STNDADVGGS SKADLTSRMV RWRNSYMVEE DVLWRLARAV N HGTGIKTS ...文字列: MVTVTDPLTA RLEAAIKAWS DFFSDAEHER LDPAIFADQS QTLFANHPLA PVPLADLLLR PTPSNRECVD QRTLQYLQVL QKQGRITTA AVLRALYKYS TAHTRAQTPD GKPKHGAGDS STNDADVGGS SKADLTSRMV RWRNSYMVEE DVLWRLARAV N HGTGIKTS HDVTEVAKVL ARWTALFAEV SAAISRDAFN SMNGLQVKDE SEDARNAFVL FYFAFCENQI VNETLSQPVC KD ICRKLLD SLDAFLPTLM HLTADITGRL EHFRSEVLAR YAPQEKKSMD MPSFMNDLSM SLESFQVPEL PVVNTRAGLY IYL GAALVG RPMIDDEALF SYLHNRYQGD LQAMAVHLIL ASFDLLANAV FRNEGAKTGH LLKSFLINKV PLILVQLVAY AATT MYPFN AEMCITEALN QVDINMFPTL SGMFDMPNNN SFNDSVRQDF CFACQLHGLL SQAAIETLLG DITYQSLPPE GRYVK EQLV QACLQEPDRT LKLIGELDNM NGNVGAAAQA IVEICRDLAS KPLSLDVLLL FDKPHKILHP LCELLDNWAG YEEDHG EYQ PVYEEFGSVL LLLLAFVYRY NLSTADLGIR SSGSFVAKLL NGVDRCQPLE QLSEQEKSHL GGWIHGLFDT EAGGLGD EL MSSCPPQDFY LLAPTLFHQI VNALSAGYLT DEMLKGGLEY LVDVLLLPAL VPALLYLSNL LWADNQPIQN AVIKILQP I LKPTSISNEA STMLSSVLNI VAKPLEHALK SYQRQDPECQ KIEPLLLAIA DNLAVSRRTG GADHTELESW CSAQITNPA TGALIHGGLA AAVRTTIQQL VQWAQNPTLN SMNGMPAPYT HRQTLAAQQI LGPHRLLGII LDELKSSPEP GIAYDVVTTM ICAPDVRNS TISSPSTQSN NSSDHNDQAQ NHQSQDAKHK HPHRLTLRDA LRLEAHDFRA HLRADPVLAE TVVRLYRRVE A QLTPLALP LPPAAAAAPA VGVNVGVDAA TAAAAAAAAA MMPDALGLGV VGGVELGGME GAIAAAVAAA NGSGTGGAGG DG TQGGAGD AGMGLDGQQQ GQGGSSAGDM GLGGGTADDI FSGLSGPDDF GADFGSWSMD LS UniProtKB: Mediator of RNA polymerase II transcription subunit 5 |
-分子 #2: Mediator of RNA polymerase II transcription subunit 14
分子 | 名称: Mediator of RNA polymerase II transcription subunit 14 タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類) 株: DSM 1495 / CBS 144.50 / IMI 039719 |
分子量 | 理論値: 133.223094 KDa |
組換発現 | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
配列 | 文字列: MEARAHGALP NNYDRERFIN GVGGDDKALK RKLEEPLSDI TKELDTVIAQ SDVAVADPKG QLCPDADVPD EMEHITDGIL PLNLLLIRL AEFSHSKLEE LVTMLASKPV PQHAVNGNGS HSTLVEDASP ESQEKKRLLL NTIQDLHSRW VKALVITEWA R NAEKVGKL ...文字列: MEARAHGALP NNYDRERFIN GVGGDDKALK RKLEEPLSDI TKELDTVIAQ SDVAVADPKG QLCPDADVPD EMEHITDGIL PLNLLLIRL AEFSHSKLEE LVTMLASKPV PQHAVNGNGS HSTLVEDASP ESQEKKRLLL NTIQDLHSRW VKALVITEWA R NAEKVGKL IDIRTHLFKK LELYPQVLND FINLKRDMAW AKVPSPDLKT ALHILTHGEV TWMPDFNFLD PPPLTTEETL RW INDMNLA LHARLQLEEH DKLPPPFKNY TIDSGRVTFR VRGEFEVDLS ISEEDFSEQF WFINFRFDFS PAPAELTPAV RYW MTEKVN NILKTEGLGG CYKYLHEFTL TQKIAELHRQ AIALNKGRWA NSLRVEKLNR NLGIHYWANR LHSQNLKSWI IIGV HSGED PQGLEEPKPS YLMVQWFREG VEQFIDIPFS QEKLSIEEIL EFVTAKHVGY LLFSLFRKFD GKPRFTQGKK ARLEL NVWT QKPEDYYLSM QLLDDEDLKI QVNPWMGDFI TTPSTGPWNR TLNSLGNPAE EGSKELENFR YIYIISVLKA QGKSHG WSV VRSPISQDEL RSIVHSESAS SRETFQAAWT RFVDWDPQWY AMRSMSLAGD QWWLVEVTSQ RQSISGNRLV FFTKMAP CF SEERLTDGFF NGLRDHSRRL IAEITNLREL YPGITPSQLR KLIDPTRQPP VLPVKASKIL GDFGGADAQS IAWLKDPV W LIYRGSACDT RVSDSPQQKR TQPVLDVFEA YLDVTDRRRF QTLNPRLDRD ILYNPYTGRF MFRLRAKMGV PVVPLLGIR VRQLQRLLDF LEGLRRVGDD AVPERVTLRE IVFSYGATRK RDSTNQPKVR PWRVRLDLTK EEGVGVVLEK GNPHLRVINR LEDLVNSQK FYSLATYLTL SLPLFRAFQQ LENAWQTAQE NGRGSCFILH LSLDKHTIRF VLPGHQRQIS LLIQPHEKEG K LVWEVGRP RHDPELLNEN CEFNRVLKER VWTISGSGFK GLVTGAAAEP DEGIERLLVL ISDAVLHLST SQPATAPPQP PQ AQPSQQV QQVQQQQVVS QLQPQAQQPR SAMPHASVGQ QQPHGAPAPM ARFPPQQQQI FQQQRPPQPQ PNMHGGQISQ PNM HGGPKP QLQGQHGQPG QMVAAPQQQG QRPGTGTAAG MGRSNAPLVV LD UniProtKB: Mediator of RNA polymerase II transcription subunit 14 |
-分子 #3: Mediator of RNA polymerase II transcription subunit 16
分子 | 名称: Mediator of RNA polymerase II transcription subunit 16 タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類) 株: DSM 1495 / CBS 144.50 / IMI 039719 |
分子量 | 理論値: 123.395898 KDa |
組換発現 | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
配列 | 文字列: MALLMEGGDP MSVDGSSTMP AHMRVMPDMG ITGAPMTLDD VDLFGDAVMN NALDALPPAP GHPPPSRALQ RRIAELRARG CCQGIAWSR QGTIASISAD GMSIELRFLR TNPENGDWEL TDAALSLSVA LVPASSPSAT SSSGSGTSNT TVISAGAPFV H LAWAPSVH ...文字列: MALLMEGGDP MSVDGSSTMP AHMRVMPDMG ITGAPMTLDD VDLFGDAVMN NALDALPPAP GHPPPSRALQ RRIAELRARG CCQGIAWSR QGTIASISAD GMSIELRFLR TNPENGDWEL TDAALSLSVA LVPASSPSAT SSSGSGTSNT TVISAGAPFV H LAWAPSVH TSSFELAAID APGRITIFLF TQNINKPYLS RKWDTDPVDD LHAVVGCYWL PLPFNVSYVA NWVQTDYRYE PI LSPAWGP IHPNHTKSAL VCVTTNGLLK MLFPQNNNRI EETSIELESV TASDDLITHA AIGADRNTLL IALAMGSKQL RVV RVGIQW WLPQVDKQQM PPSVPLRPSL RESRVAVTTL LDPEQQQSDG DAPIAQLTHL EILPSPLGET PQTVLPPVIL AVRS YLPQE GSLYAAHQEP FTVIDRWELF TDQPKAFHPA FEQLGAKNSA SSQPSAMQTL RKLDPVVIPG KVVVTVNTLQ FGRVV CFGF SDGTLQYRDR FTMNEVYTEQ ATTNITSPLQ VGFQWETEGP CLQMAFAPTN CSFVQVSEDG SVKWVKLRYP VDDPNM TLQ GPKLKAVAAS LAVASVGANI AGPNIHNHCD DVMAIARPLA KKFKDFPTAW VREFVTMFKI TVDYSEEAHH DQLMRNS LL QFCLSILNHF GFNGDFQPRT FSGKFANLAL AVRNIVVLIT IASNAPNTIK EKLSPLDEPE VVDALASCAK WGFDLLAW L TDRLLALSTD QTIKAMLADQ KRFPDLARYL HSKNDVSLHL LLCSSTRGLL SAVCRRLQVV ESLAHRATVY YESRYAVDP AAAAQKTLPP LYHAYVKMQR VVTASLVKAS DFDKLLTALS RDIQTAYIQT FSGVATQIRQ HASGSGGQPL TEQQQQQCNE QFIKKAQSH VELDMLLGQN PPPGFREVLA CFFGNIFPAF RATVDPRSVF FADWSLLEEI VAEDERTLKR RREGGGRYVD V FKRVELCG RGQVLPRGRI VASVKTERAE AVPMVPSQSQ SSHQAASQAQ GQQSQQQQGA TPAPNATPSV PISQLNPPTG PT QPPNSSG NTANANPNTG TNANTLGGSV PGLQGFVPVT ANLAVNASQW RRCVRCAAVM EDVWGQRPGF TFVLTQQRRC ACG GGWGLV PRGD UniProtKB: Mediator of RNA polymerase II transcription subunit 16 |
-分子 #4: MED15
分子 | 名称: MED15 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類) 株: DSM 1495 / CBS 144.50 / IMI 039719 |
分子量 | 理論値: 175.252594 KDa |
組換発現 | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
配列 | 文字列: MAANIPQFPN GQMAMLQQQQ QQAQQQAIAA NQQYMNVFIL NNLRGSGTNL PGTWQAQIPP QQLLSERFQK THILYTNIML ASNGGDPQR CAVAALNLEK QIFQNAQDKA SYDQAMAKKI SEVMKRRESN GPALQNQVIT DAQRQAQIQQ QIQLAQAQQR Q QQQLMLNR ...文字列: MAANIPQFPN GQMAMLQQQQ QQAQQQAIAA NQQYMNVFIL NNLRGSGTNL PGTWQAQIPP QQLLSERFQK THILYTNIML ASNGGDPQR CAVAALNLEK QIFQNAQDKA SYDQAMAKKI SEVMKRRESN GPALQNQVIT DAQRQAQIQQ QIQLAQAQQR Q QQQLMLNR MAAQGLTQPP QPAFQPMQNP GQVPVIPQQM GINVAVPGML PNAPSQPQLA MQMGQPRPGA IPVDLNQLSN QD RMRIHEE VMRRVNSTTE NSKMQIRQLM QQRLTPAQIS EANASGKDLV YLYFQNQVVQ VFRATMLQAQ EKAQQVAQVQ QAL LMQQRQ GIQGAGMPGV PPQGQVNPAL MNALGQQPAI SDAPMLGPNL TNEPQMGLLA QQPGQMIVPT NPAAVPGRPV TAGP QLGAI PPQPTPGNPQ APNQTPRPGQ LQQPFGMPQV NNPAAAAAAA AAAAAVQQPP QAQPGMRPPG PRTMPGQPGA ITTVA GPQP TSQSPAMNTL NAPMRQPPVA MTQATTQPVN PANAPMAGAT LNAQFNHQNN ARPLSMQGNM NNQAMAGMGA AANMNA NNN PMKEFMARLQ EQQRATAAAG FQGPKPGQVI GMPGQFPGGP MNALNQPGMF NQNSKPNAMV PPTAQAAALQ QQQQQAN AA DMANIYKIIQ TPQGQNMMNN MDIHQMILQE INKHLGGRLP PNITKWAHLR QFLAAQPNAL PSEIVQRIMS YQILQFKN F WKRASQPNAV GGIPQPVSNV PVSQFAGMNQ PVAPPPGVQV PQSISQVSPQ DIEGLRKNPK FANLTDDQLA EWIRKVKQQ SFIKKAWEIH NNQSNAAQNQ ATNSSLIGQQ KPGMTVPPTP TTAQPTSSVV QTAQSRPQGP QPTPTPQGPQ GVQPQAAQPK PTPSPAANV TTAPPATTGM SAASVQAANI RPSMPQQQQQ PAPQPQAPQP PPPQQQQQQQ QQQQQQQQQQ QQQVPQPPQN S RQAPMDPS PAMATKSLKR PSPDDSAEDT PAQQNITPMQ RAPSQAAQVA PNAPNAASGE PPKLSPELQK LRNLASEAQL EV SKEHLQP ILMTPADQQE TREKIAQVKL KMNQIRSMGL LPKWYYLTND DNRARQFFRG RFKVVKQFTD GEAMTQLRDT FTI TKEELN QYDNLLGSML RDMMMSRVNQ QQAAMMNQQQ QTQQQTTQPT PATLANQQAA ALRQAQQRVA PPPPKPVQQP PAQQ QQPHQ PAQPAQQQFQ PGVQPTPAPA PEYFGDQRLT AANLVLPPRK KPKVSGNQTS PSANQQLAAG SPQVQALSPV ATRKP EPQV KPQAQIPQQQ QQQQQQQQQQ QQQQQPQAPP APQFKCPEPG CTSLVAFPSE EALNAHRQEE HLKPAENPLA FMQEQM ASV LALDAQGRAK ASPKPSGQDV STPVPTAPPM SASLSKQGQT PRLQAATPMS RNLSMQRQGS ASGGKPGENM PTPGKSA GG KGKEGATPQQ MEDVWPQGTI DPANLFANLG GTLDAGTATI SGSIITDFAA YRSSTPKDTP ESSSSSKDSG VSEPNSDI N ENANLEIDLF WSNIDDGLLM DINNISMDGA AGSMGGSSGN AGGLVDISQF VEDPWGVDSA NFSLDDFGRE LDKGFGFLD GTLGEGQQES GQGQGQGQPE VMVIE UniProtKB: Uncharacterized protein |
-分子 #5: Unknown peptide
分子 | 名称: Unknown peptide / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
分子量 | 理論値: 4.443468 KDa |
組換発現 | 生物種: Chaetomium thermophilum var. thermophilum DSM 1495 (菌類) |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: OTHER / 撮影モード: OTHER |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 60.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |