National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM108753
米国
引用
ジャーナル: Sci Adv / 年: 2020 タイトル: The structures of natively assembled clathrin-coated vesicles. 著者: Mohammadreza Paraan / Joshua Mendez / Savanna Sharum / Danielle Kurtin / Huan He / Scott M Stagg / 要旨: Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy ...Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
選択した数: 180000 詳細: These are subparticle images that were extracted from particle images. Each minicoat particle has 12 pentagonal faces. From a total of 15000 minicoat particles, 180000 pentagonal faces were ...詳細: These are subparticle images that were extracted from particle images. Each minicoat particle has 12 pentagonal faces. From a total of 15000 minicoat particles, 180000 pentagonal faces were extracted. The position of subparticles was calculated by the localized reconstruction script in scipion, and the subparticle images were extracted with RELION extraction tools.
モデルのタイプ: OTHER 詳細: A pentagonal face was cut out of the minicoat cage map from the same dataset.
初期 角度割当
タイプ: OTHER 詳細: Using the Euler angles of the mini-coat cage particles and the position of the pentagonal faces in the map, initial hexagonal face Eulers were calculated.