National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM108753
米国
引用
ジャーナル: Sci Adv / 年: 2020 タイトル: The structures of natively assembled clathrin-coated vesicles. 著者: Mohammadreza Paraan / Joshua Mendez / Savanna Sharum / Danielle Kurtin / Huan He / Scott M Stagg / 要旨: Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy ...Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
モデルのタイプ: OTHER 詳細: Ab initio for a small subset of particles in Relion. The ab initio model was used in a Relion classification of 60000 particles.
初期 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3)
最終 3次元分類
ソフトウェア - 名称: cisTEM (ver. 1) 詳細: Multiple rounds of 3D classification in cisTEM produced different geometries of clathrin coated vesicles. Each of which was classified until stable subsets were achieved.