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Yorodumi- PDB-1fu9: SOLUTION STRUCTURE OF THE NINTH ZINC-FINGER DOMAIN OF THE U-SHAPE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fu9 | ||||||
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Title | SOLUTION STRUCTURE OF THE NINTH ZINC-FINGER DOMAIN OF THE U-SHAPED TRANSCRIPTION FACTOR | ||||||
Components | U-SHAPED TRANSCRIPTIONAL COFACTOR | ||||||
Keywords | TRANSCRIPTION / zinc-finger / beta-hairpin + alpha-helix | ||||||
Function / homology | Function and homology information lymph gland plasmatocyte differentiation / lymph gland crystal cell differentiation / negative regulation of hemocyte differentiation / amnioserosa maintenance / positive regulation of antibacterial peptide biosynthetic process / germ-band shortening / Factors involved in megakaryocyte development and platelet production / larval lymph gland hemopoiesis / lymph gland development / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function ...lymph gland plasmatocyte differentiation / lymph gland crystal cell differentiation / negative regulation of hemocyte differentiation / amnioserosa maintenance / positive regulation of antibacterial peptide biosynthetic process / germ-band shortening / Factors involved in megakaryocyte development and platelet production / larval lymph gland hemopoiesis / lymph gland development / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / compound eye development / chaeta development / DNA-binding transcription repressor activity / negative regulation of insulin receptor signaling pathway / heart development / RNA polymerase II-specific DNA-binding transcription factor binding / cell differentiation / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / metal ion binding / nucleus Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / molecular dynamics simulated annealing torsion angle dynamics | ||||||
Authors | Liew, C.K. / Kowalski, K. / Fox, A.H. / Newton, A. / Sharpe, B.K. / Crossley, M. / Mackay, J.P. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 2000 Title: Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions. Authors: Liew, C.K. / Kowalski, K. / Fox, A.H. / Newton, A. / Sharpe, B.K. / Crossley, M. / Mackay, J.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fu9.cif.gz | 228.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fu9.ent.gz | 194.8 KB | Display | PDB format |
PDBx/mmJSON format | 1fu9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fu/1fu9 ftp://data.pdbj.org/pub/pdb/validation_reports/fu/1fu9 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4174.862 Da / Num. of mol.: 1 / Fragment: NINTH ZINC-FINGER DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Plasmid: PGEX-2T / Production host: Escherichia coli (E. coli) / References: UniProt: Q9VPQ6 |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using a combination of 2D homonuclear and 3D 15N-heteronuclear techniques |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics simulated annealing torsion angle dynamics Software ordinal: 1 Details: The structures are based on a total of 535 restraints, 506 are NOE-derived distance constraints, 29 are dihedral angle restraints | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 500 / Conformers submitted total number: 20 |