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Yorodumi- PDB-1a0b: HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERIC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a0b | ||||||
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Title | HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI | ||||||
Components | AEROBIC RESPIRATION CONTROL SENSOR PROTEIN ARCB | ||||||
Keywords | HISTIDINE KINASE / PHOSPHOTRANSFER / TWO-COMPONENT SYSTEM / FOUR-HELIX BUNDLE | ||||||
Function / homology | Function and homology information peptidyl-histidine phosphorylation / response to oxygen levels / histidine kinase / phosphorelay sensor kinase activity / plasma membrane => GO:0005886 / phosphoprotein phosphatase activity / protein autophosphorylation / regulation of DNA-templated transcription / signal transduction / ATP binding ...peptidyl-histidine phosphorylation / response to oxygen levels / histidine kinase / phosphorelay sensor kinase activity / plasma membrane => GO:0005886 / phosphoprotein phosphatase activity / protein autophosphorylation / regulation of DNA-templated transcription / signal transduction / ATP binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.06 Å | ||||||
Authors | Kato, M. / Mizuno, T. / Shimizu, T. / Hakoshima, T. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1997 Title: Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB. Authors: Kato, M. / Mizuno, T. / Shimizu, T. / Hakoshima, T. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1996 Title: Crystallization and Preliminary X-Ray Analysis of a Histidine Kinase Domain of the Anaerobic Sensor Protein Arcb from Escherichia Coli Authors: Kato, M. / Ishige, K. / Mizuno, T. / Shimizu, T. / Hakoshima, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a0b.cif.gz | 38.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a0b.ent.gz | 25.7 KB | Display | PDB format |
PDBx/mmJSON format | 1a0b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/1a0b ftp://data.pdbj.org/pub/pdb/validation_reports/a0/1a0b | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14013.939 Da / Num. of mol.: 1 / Fragment: C-TERMINAL HPT DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PSU2DH / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli (E. coli) / Strain (production host): K-12 / Variant (production host): DZ225 References: UniProt: P22763, UniProt: P0AEC3*PLUS, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor |
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#2: Chemical | ChemComp-ZN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.1 Details: PROTEIN WAS CRYSTALLIZED FROM 12.5% PEGMME 550, 5 MM ZNSO4, 50 MM ACETIC ACID/SODIUM ACETATE BUFFER, PH 4.1 | ||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 277 K / Method: vapor diffusion, hanging dropDetails: Kato, M., (1996) Acta Crystallogr.,Sect.D, 52, 1214. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jan 1, 1996 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 2.06 Å / Num. obs: 6999 / % possible obs: 87 % / Observed criterion σ(I): 1 / Redundancy: 3.7 % / Biso Wilson estimate: 20.9 Å2 / Rmerge(I) obs: 0.045 / Net I/σ(I): 17 |
Reflection shell | Resolution: 2.06→2.25 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.11 / % possible all: 83 |
Reflection | *PLUS Num. measured all: 25850 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.06→10 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: A POSTERIORI / σ(F): 1
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Displacement parameters | Biso mean: 28 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.06→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.06→2.15 Å / Rfactor Rfree error: 0.03 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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