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    Yorodumi
    - EMDB-1843: Structural basis for the subunit assembly of the anaphase promoti... -

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    Basic information

    Entry
    Database: EMDB / ID: 1843
    TitleStructural basis for the subunit assembly of the anaphase promoting complex
    KeywordsAPC/C / anaphase promoting complex
    SampleApc4-Apc5 heterodimer
    SourceSaccharomyces cerevisiae / yeast / Baker's yeast /
    Map dataApc4 and Apc5
    Methodsingle particle reconstruction
    AuthorsSchreiber A / Stengel F / Zhang Z / Enchev RE / Kong E / Morris EP / Robinson CV / daFonseca PCA / Barford D
    CitationNature, 2011, 470, 227-232

    Nature, 2011, 470, 227-232 StrPapers
    Structural basis for the subunit assembly of the anaphase-promoting complex.
    Anne Schreiber / Florian Stengel / Ziguo Zhang / Radoslav I Enchev / Eric H Kong / Edward P Morris / Carol V Robinson / Paula C A da Fonseca / David Barford

    DateDeposition: Dec 4, 2010 / Header (metadata) release: Dec 9, 2010 / Map release: Feb 7, 2011 / Last update: Dec 4, 2010

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    Structure visualization

    Movie
    • Surface view with section colored by density value
    • Surface level: 52
    • Imaged by UCSF CHIMERA
    • Download
    • Surface view colored by radius
    • Surface level: 52
    • Imaged by UCSF CHIMERA
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    Supplemental images

    Downloads & links

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    Map

    Fileemd_1843.map.gz (map file in CCP4 format, 6751 KB)
    Projections & slicesSize of images:
    AxesZ (Sec.)Y (Row.)X (Col.)
    120 pix
    3.47 A/pix
    = 416.4 A
    120 pix
    3.47 A/pix
    = 416.4 A
    120 pix
    3.47 A/pix
    = 416.4 A

    Surface

    Projections

    Slices (1/3)

    Slices (1/2)

    Slices (2/3)

    Images are generated by Spider package.

    Voxel sizeX=Y=Z: 3.47 A
    Density
    Contour Level:52 (by author), 52 (movie #1):
    Minimum - Maximum-150.097 - 339.789
    Average (Standard dev.)0.606204 (9.63846)
    Details

    EMDB XML:

    Space Group Number1
    Map Geometry
    Axis orderXYZ
    Dimensions120120120
    Origin-60-60-60
    Limit595959
    Spacing120120120
    CellA=B=C: 416.4 A
    Alpha=beta=gamma: 90 deg.

    CCP4 map header:

    modeImage stored as Reals
    A/pix X/Y/Z3.473.473.47
    M x/y/z120120120
    origin x/y/z0.0000.0000.000
    length x/y/z416.400416.400416.400
    alpha/beta/gamma90.00090.00090.000
    start NX/NY/NZ-30-24-70
    NX/NY/NZ6149141
    MAP C/R/S123
    start NC/NR/NS-60-60-60
    NC/NR/NS120120120
    D min/max/mean-150.097339.7890.606

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    Supplemental data

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    Sample components

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    Entire Apc4-Apc5 heterodimer

    EntireName: Apc4-Apc5 heterodimer / Number of components: 1
    MassTheoretical: 160 kDa / Experimental: 155 kDa

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    Component #1: protein, Apc4_5

    ProteinName: Apc4_5 / a.k.a: Apc4_5 / Recombinant expression: Yes
    SourceSpecies: Saccharomyces cerevisiae / yeast / Baker's yeast /

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    Experimental details

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    Sample preparation

    Specimen stateparticle
    VitrificationInstrument: NONE / Cryogen name: NONE

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    Electron microscopy imaging

    ImagingMicroscope: FEI TECNAI F20
    Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
    LensImaging mode: BRIGHT FIELD
    Specimen HolderHolder: Eucentric / Model: OTHER

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    Image processing

    ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric)

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