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- EMDB-1203: An expanded protein folding cage in the GroEL-gp31 complex. -

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Basic information

Entry
Database: EMDB / ID: EMD-1203
TitleAn expanded protein folding cage in the GroEL-gp31 complex.
Map dataGroEL-ATP-gp31 3D density map
Sample
  • Sample: GroEL-ATP-gp31
  • Protein or peptide: GroEL
  • Protein or peptide: gp31
  • Ligand: ATPAdenosine triphosphate
Function / homology: / protein binding / Chaperonin Cpn60/GroEL
Function and homology information
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 12.0 Å
AuthorsClare DK / Bakkes PJ / van Heerikhuizen H / van der Vies SM / Saibil HR
CitationJournal: J Mol Biol / Year: 2006
Title: An expanded protein folding cage in the GroEL-gp31 complex.
Authors: Daniel K Clare / Patrick J Bakkes / Harm van Heerikhuizen / Saskia M van der Vies / Helen R Saibil /
Abstract: Bacteriophage T4 produces a GroES analogue, gp31, which cooperates with the Escherichia coli GroEL to fold its major coat protein gp23. We have used cryo-electron microscopy and image processing to ...Bacteriophage T4 produces a GroES analogue, gp31, which cooperates with the Escherichia coli GroEL to fold its major coat protein gp23. We have used cryo-electron microscopy and image processing to obtain three-dimensional structures of the E.coli chaperonin GroEL complexed with gp31, in the presence of both ATP and ADP. The GroEL-gp31-ADP map has a resolution of 8.2 A, which allows accurate fitting of the GroEL and gp31 crystal structures. Comparison of this fitted structure with that of the GroEL-GroES-ADP structure previously determined by cryo-electron microscopy shows that the folding cage is expanded. The enlarged volume for folding is consistent with the size of the bacteriophage coat protein gp23, which is the major substrate of GroEL-gp31 chaperonin complex. At 56 kDa, gp23 is close to the maximum size limit of a polypeptide that is thought to fit inside the GroEL-GroES folding cage.
History
DepositionMar 6, 2006-
Header (metadata) releaseMar 9, 2006-
Map releaseMar 9, 2006-
UpdateMay 26, 2011-
Current statusMay 26, 2011Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.27
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.27
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

1203.gif

Downloads & links

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Map

FileDownload / File: emd_1203.map.gz / Format: CCP4 / Size: 26.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationGroEL-ATP-gp31 3D density map
Voxel sizeX=Y=Z: 1.4 Å
Density
Contour Level1: 0.749 / Movie #1: 0.27
Minimum - Maximum-3.13969 - 5.33992
Average (Standard dev.)0.265517 (±0.481641)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions192192192
Spacing192192192
CellA=B=C: 268.8 Å
α=β=γ: 90 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.41.41.4
M x/y/z192192192
origin x/y/z0.0000.0000.000
length x/y/z268.800268.800268.800
α/β/γ90.00090.00090.000
start NX/NY/NZ-96-96-96
NX/NY/NZ192192192
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS192192192
D min/max/mean-3.1405.3400.266

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Supplemental data

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Sample components

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Entire : GroEL-ATP-gp31

EntireName: GroEL-ATP-gp31
Components
  • Sample: GroEL-ATP-gp31
  • Protein or peptide: GroEL
  • Protein or peptide: gp31
  • Ligand: ATPAdenosine triphosphate

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Supramolecule #1000: GroEL-ATP-gp31

SupramoleculeName: GroEL-ATP-gp31 / type: sample / ID: 1000
Oligomeric state: heptamer of gp31 and a tetradecamer of GroEL
Number unique components: 3
Molecular weightExperimental: 900 KDa / Theoretical: 900 KDa / Method: Mass spectrometry

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Macromolecule #1: GroEL

MacromoleculeName: GroEL / type: protein_or_peptide / ID: 1 / Name.synonym: chaperonin / Number of copies: 1 / Oligomeric state: tetradecamer / Recombinant expression: Yes
Source (natural)Organism: Escherichia coli (E. coli) / Strain: E. coli MC1009 and BL21 / synonym: E. coli / Location in cell: cytoplasm
Molecular weightExperimental: 800 KDa / Theoretical: 800 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant plasmid: pSL6
SequenceGO: protein binding / InterPro: Chaperonin Cpn60/GroEL

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Macromolecule #2: gp31

MacromoleculeName: gp31 / type: protein_or_peptide / ID: 2 / Name.synonym: co-chaperonin / Number of copies: 1 / Oligomeric state: heptamer / Recombinant expression: Yes
Source (natural)Organism: Escherichia coli (E. coli) / synonym: E. coli / Location in cell: cytoplasm
Molecular weightExperimental: 100 KDa / Theoretical: 100 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant plasmid: pAR1
SequenceInterPro: INTERPRO: IPR011597

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Macromolecule #3: ATP

MacromoleculeName: ATP / type: ligand / ID: 3 / Name.synonym: nucleotide / Recombinant expression: No

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.4 / Details: 20mM Tris-HCL, 10mM MgCl, 10mM KCl
GridDetails: 300 mesh copper grid - holey carbon film
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 100 K / Instrument: HOMEMADE PLUNGER / Details: Vitrification instrument: home made / Timed resolved state: Vitrified after 5 minute incubation
Method: The grids were bloted for 2-3 seconds and then left to equilibrate for 2-3 seconds and then plunged into liquid ethane

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Electron microscopy

MicroscopeFEI TECNAI F20
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 50000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 3.1 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 50000
Sample stageSpecimen holder: single tilt / Specimen holder model: GATAN LIQUID NITROGEN
TemperatureAverage: 100 K
Alignment procedureLegacy - Astigmatism: objective lens astigmatism was corrected at 150kX
DateSep 1, 2004
Image recordingCategory: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Digitization - Sampling interval: 7 µm / Number real images: 14 / Average electron dose: 15 e/Å2 / Od range: 1 / Bits/pixel: 8
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

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Image processing

CTF correctionDetails: Each particle
Final two d classificationNumber classes: 166
Final angle assignmentDetails: theta 15 (88-103), phi 51(0-51)
Final reconstructionApplied symmetry - Point group: C7 (7 fold cyclic) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 12.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: spider and imagic
Details: final maps were calculated from 166 averaged angle bins
Number images used: 2500
DetailsThe particles were hand picked

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