登録情報 データベース : EMDB / ID : EMD-11893 構造の表示 ダウンロードとリンクタイトル 43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase マップデータ 詳細 試料複合体 : 43S preinitiation complex from Trypanosoma cruzi with the helicase kDDX60タンパク質・ペプチド : x 50種RNA : x 2種リガンド : x 3種 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
: / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / regulation of translational initiation / translation regulator activity / translation initiation factor binding / translation initiation factor activity ... : / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / regulation of translational initiation / translation regulator activity / translation initiation factor binding / translation initiation factor activity / metallopeptidase activity / ribosomal small subunit assembly / ribosome binding / kinase activity / small ribosomal subunit / cytosolic small ribosomal subunit / tRNA binding / nucleic acid binding / membrane => GO:0016020 / rRNA binding / リボソーム / structural constituent of ribosome / ribonucleoprotein complex / 翻訳 (生物学) / リン酸化 / GTPase activity / GTP binding / RNA binding / zinc ion binding / ATP binding / metal ion binding / 細胞質 類似検索 - 分子機能 Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic ... Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor SUI1 / SUI1 domain superfamily / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Eukaryotic translation initiation factor 3 subunit A / SUI1 domain / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / W2 domain / W2 domain profile. / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / RNA-binding domain, S1 / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Ribosomal protein L41 / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S2, eukaryotic / S1 domain profile. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S12e signature. / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S12e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S4, C-terminal domain / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S19e / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / 40S Ribosomal protein S10 / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / RS4NT (NUC023) domain / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal family S4e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S8e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes 類似検索 - ドメイン・相同性 40S ribosomal protein S33 / Putative 40S ribosomal protein S21 / 60S ribosomal protein L40 / Putative 40S ribosomal protein S9 / Putative eukaryotic translation initiation factor 1A / Uncharacterized protein / 40S ribosomal protein S4 / Eukaryotic translation initiation factor 2 subunit, putative / 40S ribosomal protein SA / 40S ribosomal protein S12 ... 40S ribosomal protein S33 / Putative 40S ribosomal protein S21 / 60S ribosomal protein L40 / Putative 40S ribosomal protein S9 / Putative eukaryotic translation initiation factor 1A / Uncharacterized protein / 40S ribosomal protein S4 / Eukaryotic translation initiation factor 2 subunit, putative / 40S ribosomal protein SA / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S15a, putative / 40S ribosomal protein S26 / 40S ribosomal protein S11, putative / Eukaryotic translation initiation factor 3 (EIF-3) interacting protein, putative / Ribosomal protein S20, putative / 40S ribosomal protein S14, putative / Eukaryotic translation initiation factor 3 subunit E / 40S ribosomal protein S30 / 40S ribosomal protein S13, putative / Eukaryotic translation initiation factor 3 subunit 7-like protein, putative / CSN8_PSD8_EIF3K domain-containing protein / 40S ribosomal protein S16, putative / 40S ribosomal protein S15, putative / Translation initiation factor, putative / 40S ribosomal protein S2, putative / Ribosomal protein S19, putative / 40S ribosomal protein S17, putative / Uncharacterized protein / Protein translation factor SUI1 homolog, putative / 40S ribosomal protein S3, putative / 40S ribosomal protein S27, putative / JAB_MPN domain-containing protein / Uncharacterized protein / 40S ribosomal protein S6 / Guanine nucleotide-binding protein subunit beta-like protein / 40S ribosomal protein S23, putative / 40S ribosomal protein S25 / 40S ribosomal protein S8 / 40S ribosomal protein S24 / RNA-binding protein, putative / 40S ribosomal protein S3a-2 / Eukaryotic translation initiation factor 5, putative / 40S ribosomal protein S10, putative / 40S ribosomal protein S18, putative / 40S ribosomal protein S5, putative / Eukaryotic translation initiation factor 3 subunit 8, putative / ATP-dependent DEAD/H RNA helicase / Elongation initiation factor 2 alpha subunit, putative 類似検索 - 構成要素生物種 Trypanosoma cruzi (クルーズトリパノソーマ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.33 Å 詳細 データ登録者Bochler A / Brito Querido J / Prilepskaja T / Soufari H / Del Cistia ML / Kuhn L / Rimoldi Ribeiro A / Valasek LS / Hashem Y 資金援助 フランス, チェコ, 4件 詳細 詳細を隠すOrganization Grant number 国 European Research Council (ERC) ERC-2017-STG#759120TransTryp フランス Laboratories of Excellence (LabEx) ANR10LABX0036NETRNA フランス Laboratories of Excellence (LabEx) ANR 14 ACHN0024 CryoEM80S フランス Czech Science Foundation Grant of Excellence in Basic Research (EXPRO2019) チェコ
引用ジャーナル : Cell Rep / 年 : 2020タイトル : Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.著者 : Anthony Bochler / Jailson Brito Querido / Terezie Prilepskaja / Heddy Soufari / Angelita Simonetti / Mayara Lucia Del Cistia / Lauriane Kuhn / Aline Rimoldi Ribeiro / Leoš Shivaya Valášek / Yaser Hashem / 要旨 : Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA and several eukaryotic initiation ... Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9, 7, and 6, and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data. 履歴 登録 2020年10月27日 - ヘッダ(付随情報) 公開 2021年4月14日 - マップ公開 2021年4月14日 - 更新 2021年4月21日 - 現状 2021年4月21日 処理サイト : PDBe / 状態 : 公開
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