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Structure paper

TitleStructure of an insect gustatory receptor.
Journal, issue, pagesbioRxiv, Year 2023
Publish dateDec 19, 2023
AuthorsHeather M Frank / Sanket Walujkar / Richard M Walsh / Willem J Laursen / Douglas L Theobald / Paul A Garrity / Rachelle Gaudet /
PubMed AbstractGustatory Receptors (GRs) are critical for insect chemosensation and are potential targets for controlling pests and disease vectors. However, GR structures have not been experimentally determined. ...Gustatory Receptors (GRs) are critical for insect chemosensation and are potential targets for controlling pests and disease vectors. However, GR structures have not been experimentally determined. We present structures of Gr9 (BmGr9), a fructose-gated cation channel, in agonist-free and fructose-bound states. BmGr9 forms a tetramer similar to distantly related insect Olfactory Receptors (ORs). Upon fructose binding, BmGr9's ion channel gate opens through helix S7b movements. In contrast to ORs, BmGR9's ligand-binding pocket, shaped by a kinked helix S4 and a shorter extracellular S3-S4 loop, is larger and solvent accessible in both agonist-free and fructose-bound states. Also unlike ORs, fructose binding by BmGr9 involves helix S5 and a binding pocket lined with aromatic and polar residues. Structure-based sequence alignments reveal distinct patterns of ligand-binding pocket residue conservation in GR subfamilies associated with distinct ligand classes. These data provide insight into the molecular basis of GR ligand specificity and function.
External linksbioRxiv / PubMed:38187590 / PubMed Central
MethodsEM (single particle)
Resolution2.85 - 3.98 Å
Structure data

43129

8vc1

EMDB-43129, PDB-8vc1:
CryoEM structure of insect gustatory receptor BmGr9
Method: EM (single particle) / Resolution: 2.85 Å

43130

8vc2

EMDB-43130, PDB-8vc2:
CryoEM structure of insect gustatory receptor BmGr9 in the presence of fructose
Method: EM (single particle) / Resolution: 3.98 Å

Chemicals

ChemComp-PSC:
(7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSA-17,20-DIEN-1-AMINIUM 4-OXIDE / phospholipid*YM / Phosphatidylcholine

ChemComp-PC7:
(7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipid*YM / Phosphatidylcholine

ChemComp-9Z9:
(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en / detergent*YM

Source
  • bombyx mori (domestic silkworm)
KeywordsMEMBRANE PROTEIN / gustatory receptor / homotetramer / ligand-gated ion channel / seven transmembrane

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