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TitleMolecular basis of human polyA polymerase recruitment by mPSF.
Journal, issue, pagesRNA, Year 2024
Publish dateMar 27, 2024
AuthorsSofia Todesca / Felix Sandmeir / Achim Keidel / Elena Conti
PubMed Abstract3' end processing of most eukaryotic pre-mRNAs is a crucial co-transcriptional process that generally involves the cleavage and polyadenylation of the precursor transcripts. Within the human 3' end ...3' end processing of most eukaryotic pre-mRNAs is a crucial co-transcriptional process that generally involves the cleavage and polyadenylation of the precursor transcripts. Within the human 3' end processing machinery, the 4-subunit mammalian polyadenylation specificity factor (mPSF) recognizes the polyadenylation signal (PAS) in the pre-mRNA and recruits the polyA polymerase α (PAPOA) to it. To shed light on the molecular mechanisms of PAPOA recruitment to mPSF, we used a combination of cryogenic-electron microscopy (cryo-EM) single-particle analysis, computational structure prediction and biochemistry to reveal an intricate interaction network. A short linear motif in the mPSF subunit FIP1 interacts with the structured core of human PAPOA, with a binding mode that is evolutionary conserved from yeast to human. In higher eukaryotes, however, PAPOA contains a conserved C-terminal motif that can interact intramolecularly with the same residues of the PAPOA structured core used to bind FIP1. Interestingly, using biochemical assay and cryo-EM structural analysis, we found that the PAPOA C-terminal motif can also directly interact with mPSF at the subunit CPSF160. These results show that PAPOA recruitment to mPSF is mediated by two distinct intermolecular connections and further suggest the presence of mutually exclusive interactions in the regulation of 3' end processing.
External linksRNA / PubMed:38538052
MethodsEM (single particle)
Resolution2.79 Å
Structure data

19008

8r8r

EMDB-19008, PDB-8r8r:
Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Method: EM (single particle) / Resolution: 2.79 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / 3-end processing / polyadenylation / PAPOA / pre-mRNA / cleavage and polyadenylation

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