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TitleVisualizing chaperone-mediated multistep assembly of the human 20S proteasome.
Journal, issue, pagesNat Struct Mol Biol, Year 2024
Publish dateApr 10, 2024
AuthorsFrank Adolf / Jiale Du / Ellen A Goodall / Richard M Walsh / Shaun Rawson / Susanne von Gronau / J Wade Harper / John Hanna / Brenda A Schulman /
PubMed AbstractDedicated assembly factors orchestrate the stepwise production of many molecular machines, including the 28-subunit proteasome core particle (CP) that mediates protein degradation. Here we report ...Dedicated assembly factors orchestrate the stepwise production of many molecular machines, including the 28-subunit proteasome core particle (CP) that mediates protein degradation. Here we report cryo-electron microscopy reconstructions of seven recombinant human subcomplexes that visualize all five chaperones and the three active site propeptides across a wide swath of the assembly pathway. Comparison of these chaperone-bound intermediates and a matching mature CP reveals molecular mechanisms determining the order of successive subunit additions, as well as how proteasome subcomplexes and assembly factors structurally adapt upon progressive subunit incorporation to stabilize intermediates, facilitate the formation of subsequent intermediates and ultimately rearrange to coordinate proteolytic activation with gated access to active sites. This work establishes a methodologic approach for structural analysis of multiprotein complex assembly intermediates, illuminates specific functions of assembly factors and reveals conceptual principles underlying human proteasome biogenesis, thus providing an explanation for many previous biochemical and genetic observations.
External linksNat Struct Mol Biol / PubMed:38600324
MethodsEM (single particle)
Resolution2.67 - 4.17 Å
Structure data

18755

8qyj

EMDB-18755, PDB-8qyj:
Human 20S proteasome assembly structure 1
Method: EM (single particle) / Resolution: 2.73 Å

18757

8qyl

EMDB-18757, PDB-8qyl:
Human 20S proteasome assembly intermediate structure 2
Method: EM (single particle) / Resolution: 2.67 Å

18758

8qym

EMDB-18758, PDB-8qym:
Human 20S proteasome assembly intermediate structure 3
Method: EM (single particle) / Resolution: 2.73 Å

18759

8qyn

EMDB-18759, PDB-8qyn:
Human 20S proteasome assembly intermediate structure 5
Method: EM (single particle) / Resolution: 2.88 Å

18760

8qyo

EMDB-18760, PDB-8qyo:
Human proteasome 20S core particle
Method: EM (single particle) / Resolution: 2.84 Å

18761

8qys

EMDB-18761, PDB-8qys:
Human preholo proteasome 20S core particle
Method: EM (single particle) / Resolution: 3.89 Å

EMDB-18762: Human premature proteasome 20S core particle
Method: EM (single particle) / Resolution: 3.92 Å

18773

8qz9

EMDB-18773, PDB-8qz9:
Human 20S proteasome assembly intermediate structure 4
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-19342: Human 20S proteasome assembly intermediate structure 5 beta7 tagged
Method: EM (single particle) / Resolution: 4.17 Å

EMDB-19343: Human 20S core particle beta7 tagged
Method: EM (single particle) / Resolution: 3.22 Å

Source
  • homo sapiens (human)
KeywordsHYDROLASE / Complex

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