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TitleA marine cryptochrome with an inverse photo-oligomerization mechanism.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 6918, Year 2023
Publish dateOct 30, 2023
AuthorsHong Ha Vu / Heide Behrmann / Maja Hanić / Gayathri Jeyasankar / Shruthi Krishnan / Dennis Dannecker / Constantin Hammer / Monika Gunkel / Ilia A Solov'yov / Eva Wolf / Elmar Behrmann /
PubMed AbstractCryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, ...Cryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, its light receptive cryptochrome L-CRY (PdLCry) allows the animal to discriminate between sunlight and moonlight, an important requirement for synchronizing its lunar cycle-dependent mass spawning. Using cryo-electron microscopy, we show that in the dark, PdLCry adopts a dimer arrangement observed neither in plant nor insect CRYs. Intense illumination disassembles the dimer into monomers. Structural and functional data suggest a mechanistic coupling between the light-sensing flavin adenine dinucleotide chromophore, the dimer interface, and the C-terminal tail helix, with a likely involvement of the phosphate binding loop. Taken together, our work establishes PdLCry as a CRY protein with inverse photo-oligomerization with respect to plant CRYs, and provides molecular insights into how this protein might help discriminating the different light intensities associated with sunlight and moonlight.
External linksNat Commun / PubMed:37903809 / PubMed Central
MethodsEM (single particle)
Resolution2.57 - 8.0 Å
Structure data

17429

8p4x

EMDB-17429, PDB-8p4x:
FAD_ox bound dark state structure of PdLCry
Method: EM (single particle) / Resolution: 2.57 Å

EMDB-17533: FAD_AQ bound blue-light state structure of PdLCry
Method: EM (single particle) / Resolution: 8.0 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • platynereis dumerilii (Dumeril's clam worm)
KeywordsFLAVOPROTEIN / light-sensitive / circalunar clock

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