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TitleStructure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na/H antiporter.
Journal, issue, pagesNat Plants, Vol. 9, Issue 11, Page 1924-1936, Year 2023
Publish dateOct 26, 2023
AuthorsXiang-Yun Zhang / Ling-Hui Tang / Jia-Wei Nie / Chun-Rui Zhang / Xiaonan Han / Qi-Yu Li / Li Qin / Mei-Hua Wang / Xiahe Huang / Feifei Yu / Min Su / Yingchun Wang / Rui-Ming Xu / Yan Guo / Qi Xie / Yu-Hang Chen /
PubMed AbstractSalinity is one of the most severe abiotic stresses that adversely affect plant growth and agricultural productivity. The plant Na/H antiporter Salt Overly Sensitive 1 (SOS1) located in the plasma ...Salinity is one of the most severe abiotic stresses that adversely affect plant growth and agricultural productivity. The plant Na/H antiporter Salt Overly Sensitive 1 (SOS1) located in the plasma membrane extrudes excess Na out of cells in response to salt stress and confers salt tolerance. However, the molecular mechanism underlying SOS1 activation remains largely elusive. Here we elucidate two cryo-electron microscopy structures of rice (Oryza sativa) SOS1, a full-length protein in an auto-inhibited state and a truncated version in an active state. The SOS1 forms a dimeric architecture, with an NhaA-folded transmembrane domain portion in the membrane and an elongated cytosolic portion of multiple regulatory domains in the cytoplasm. The structural comparison shows that SOS1 adopts an elevator transport mechanism accompanied by a conformational transition of the highly conserved Pro in the unwound transmembrane helix 5 (TM), switching from an occluded conformation in the auto-inhibited state to a conducting conformation in the active state. These findings allow us to propose an inhibition-release mechanism for SOS1 activation and elucidate how SOS1 controls Na homeostasis in response to salt stress.
External linksNat Plants / PubMed:37884653
MethodsEM (single particle)
Resolution3.09 - 3.4 Å
Structure data

35775

8iwo

EMDB-35775, PDB-8iwo:
The rice Na+/H+ antiporter SOS1 in an auto-inhibited state
Method: EM (single particle) / Resolution: 3.09 Å

35950

8j2m

EMDB-35950, PDB-8j2m:
The truncated rice Na+/H+ antiporter SOS1 (1-976) in a constitutively active state
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-46E:
(2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl tetradecanoate

Source
  • Oryza sativa (Asian cultivated rice)
  • oryza sativa japonica group (Japanese rice)
KeywordsMEMBRANE PROTEIN / Na+/H+ antiporter / salt stress / cation:proton antiporter family 1

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