Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Structure of the transcription open complex of distinct σ factors. |
|---|---|
| Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 6455, Year 2023 |
| Publish date | Oct 13, 2023 |
 Authors | Jie Li / Haonan Zhang / Dongyu Li / Ya-Jun Liu / Edward A Bayer / Qiu Cui / Yingang Feng / Ping Zhu /   |
| PubMed Abstract | Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ ...Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ paralogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σ factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium, Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σ paralogues were further revealed by comparison of the two structures. Consequently, the σ factors represent a distinct set of the σ-family σ factors, thus highlighting the diversity of bacterial transcription. |
 External links | Nat Commun / PubMed:37833284 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.03 - 3.36 Å |
| Structure data | EMDB-35130, PDB-8i23: EMDB-35131, PDB-8i24: |
| Chemicals |  ChemComp-ZN:  ChemComp-MG: |
| Source |
|
 Keywords | TRANSCRIPTION/DNA / TRANSCRIPTION OPEN COMPLEX / SIGI /  TRANSCRIPTION / TRANSCRIPTION-DNA complex |
