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TitleStructural and functional properties of different types of siphonous LHCII trimers from an intertidal green alga Bryopsis corticulans.
Journal, issue, pagesStructure, Vol. 31, Issue 10, Page 1247-11258.e3, Year 2023
Publish dateOct 5, 2023
AuthorsZhenhua Li / Cuicui Zhou / Songhao Zhao / Jinyang Zhang / Xueyang Liu / Min Sang / Xiaochun Qin / Yanyan Yang / Guangye Han / Tingyun Kuang / Jian-Ren Shen / Wenda Wang /
PubMed AbstractLight-harvesting complexes of photosystem II (LHCIIs) in green algae and plants are vital antenna apparatus for light harvesting, energy transfer, and photoprotection. Here we determined the ...Light-harvesting complexes of photosystem II (LHCIIs) in green algae and plants are vital antenna apparatus for light harvesting, energy transfer, and photoprotection. Here we determined the structure of a siphonous-type LHCII trimer from the intertidal green alga Bryopsis corticulans by X-ray crystallography and cryo-electron microscopy (cryo-EM), and analyzed its functional properties by spectral analysis. The Bryopsis LHCII (Bry-LHCII) structures in both homotrimeric and heterotrimeric form show that green light-absorbing siphonaxanthin and siphonein occupied the sites of lutein and violaxanthin in plant LHCII, and two extra chlorophylls (Chls) b replaced Chls a. Binding of these pigments expands the blue-green light absorption of B. corticulans in the tidal zone. We observed differences between the Bry-LHCII homotrimer crystal and cryo-EM structures, and also between Bry-LHCII homotrimer and heterotrimer cryo-EM structures. These conformational changes may reflect the flexibility of Bry-LHCII, which may be required to adapt to light fluctuations from tidal rhythms.
External linksStructure / PubMed:37633266
MethodsEM (single particle) / X-ray diffraction
Resolution2.55 - 2.74 Å
Structure data

34883

8hlv

EMDB-34883, PDB-8hlv:
Bry-LHCII homotrimer of Bryopsis corticulans
Method: EM (single particle) / Resolution: 2.55 Å

34930

8hpd

EMDB-34930, PDB-8hpd:
Bry-LHCII heterotrimer of Bryopsis corticulans
Method: EM (single particle) / Resolution: 2.74 Å

PDB-8hq8:
Bry-LHCII homotrimer of Bryopsis corticulans
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-0UR:
Siphonein

ChemComp-0IE:
Siphonaxanthin

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-MG:
Unknown entry

ChemComp-BET:
TRIMETHYL GLYCINE / Trimethylglycine

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-HOH:
WATER / Water

Source
  • bryopsis corticulans (plant)
KeywordsPHOTOSYNTHESIS / light-harvesting complexes / siphonaxanthin / siphonein

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