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TitleStructural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 7287, Year 2022
Publish dateNov 26, 2022
AuthorsRina Hirano / Haruhiko Ehara / Tomoya Kujirai / Tamami Uejima / Yoshimasa Takizawa / Shun-Ichi Sekine / Hitoshi Kurumizaka /
PubMed AbstractIn chromatin, linker histone H1 binds to nucleosomes, forming chromatosomes, and changes the transcription status. However, the mechanism by which RNA polymerase II (RNAPII) transcribes the DNA in ...In chromatin, linker histone H1 binds to nucleosomes, forming chromatosomes, and changes the transcription status. However, the mechanism by which RNA polymerase II (RNAPII) transcribes the DNA in the chromatosome has remained enigmatic. Here we report the cryo-electron microscopy (cryo-EM) structures of transcribing RNAPII-chromatosome complexes (forms I and II), in which RNAPII is paused at the entry linker DNA region of the chromatosome due to H1 binding. In the form I complex, the H1 bound to the nucleosome restricts the linker DNA orientation, and the exit linker DNA is captured by the RNAPII DNA binding cleft. In the form II complex, the RNAPII progresses a few bases ahead by releasing the exit linker DNA from the RNAPII cleft, and directly clashes with the H1 bound to the nucleosome. The transcription elongation factor Spt4/5 masks the RNAPII DNA binding region, and drastically reduces the H1-mediated RNAPII pausing.
External linksNat Commun / PubMed:36435862 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 4.6 Å
Structure data

34415

8h0v

EMDB-34415, PDB-8h0v:
RNA polymerase II transcribing a chromatosome (type I)
Method: EM (single particle) / Resolution: 3.8 Å

34416

8h0w

EMDB-34416, PDB-8h0w:
RNA polymerase II transcribing a chromatosome (type II)
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • komagataella phaffii (fungus)
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / chromatin / nucleosome

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