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Structure paper

TitleStructure and proposed DNA delivery mechanism of a marine roseophage.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 3609, Year 2023
Publish dateJun 17, 2023
AuthorsYang Huang / Hui Sun / Shuzhen Wei / Lanlan Cai / Liqin Liu / Yanan Jiang / Jiabao Xin / Zhenqin Chen / Yuqiong Que / Zhibo Kong / Tingting Li / Hai Yu / Jun Zhang / Ying Gu / Qingbing Zheng / Shaowei Li / Rui Zhang / Ningshao Xia /
PubMed AbstractTailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene ...Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.
External linksNat Commun / PubMed:37330604 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.43 - 11.5 Å
Structure data

34247

8gta

EMDB-34247, PDB-8gta:
Cryo-EM structure of the marine siphophage vB_Dshs-R4C capsid
Method: EM (single particle) / Resolution: 3.63 Å

34248

8gtb

EMDB-34248, PDB-8gtb:
Cryo-EM structure of the marine siphophage vB_DshS-R4C tail tube protein
Method: EM (helical sym.) / Resolution: 3.43 Å

34249

8gtc

EMDB-34249, PDB-8gtc:
Cryo-EM model of the marine siphophage vB_DshS-R4C baseplate-tail complex
Method: EM (single particle) / Resolution: 4.5 Å

34250

8gtd

EMDB-34250, PDB-8gtd:
Cryo-EM model of the marine siphophage vB_DshS-R4C portal-adaptor complex
Method: EM (single particle) / Resolution: 4.7 Å

34252

8gtf

EMDB-34252, PDB-8gtf:
Cryo-EM model of the marine siphophage vB_DshS-R4C stopper-terminator complex
Method: EM (single particle) / Resolution: 6.6 Å

EMDB-34253: Dinoroseobacter phage vB_DshS-R4C C1 portal vertex
Method: EM (single particle) / Resolution: 11.5 Å

EMDB-34254: Dinoroseobacter phage vB_DshS-R4C C5 portal vertex
Method: EM (single particle) / Resolution: 6.6 Å

Source
  • dinoroseobacter phage vb_dshs-r4c (virus)
KeywordsVIRAL PROTEIN / Marine bacteriophage / Cyro-EM / Siphophage / capsid / Cryo-EM / tail tube protein / Baseplate / Megatron protein / Tail fibre protein / Distal tail protein / Hub protein / VIRUS / Portal protein / Adaptor protein / Head-to-tail interface / Stopper protein / Terminator protein

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