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TitleHIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation.
Journal, issue, pagesJ Mol Biol, Vol. 435, Issue 15, Page 168143, Year 2023
Publish dateMay 6, 2023
AuthorsNathaniel Talledge / Huixin Yang / Ke Shi / Raffaele Coray / Guichuan Yu / William G Arndt / Shuyu Meng / Gloria C Baxter / Luiza M Mendonça / Daniel Castaño-Díez / Hideki Aihara / Louis M Mansky / Wei Zhang /
PubMed AbstractRetrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus ...Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used the single particle cryo-electron microscopy method to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag lattice structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation.
External linksJ Mol Biol / PubMed:37150290 / PubMed Central
MethodsEM (tomography) / EM (single particle) / X-ray diffraction
Resolution1.98 - 9.1 Å
Structure data

EMDB-29606: CryoET of HIV-2 Immature Particles
Method: EM (tomography) / Resolution: 9.1 Å

29607

8fzc

EMDB-29607, PDB-8fzc:
HIV-2 Gag Capsid from Immature Virus-like Particles
Method: EM (single particle) / Resolution: 5.5 Å

PDB-7tv2:
X-ray crystal structure of HIV-2 CA protein CTD
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Human immunodeficiency virus 2
  • human immunodeficiency virus type 2 (isolate rod)
KeywordsSTRUCTURAL PROTEIN / viral protein / VIRUS LIKE PARTICLE / Retrovirus / Lentivirus / Gag / Capsid / HIV-2 / lattice

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