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Structure paper

TitleStructural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.
Journal, issue, pagesScience, Vol. 381, Issue 6653, Page eadg4725, Year 2023
Publish dateJul 7, 2023
AuthorsShiheng Liu / Hong Wang / Xiaorun Li / Fan Zhang / Jane K J Lee / Zihang Li / Clinton Yu / Jason J Hu / Xiaojing Zhao / Takuma Suematsu / Ana L Alvarez-Cabrera / Qiushi Liu / Liye Zhang / Lan Huang / Inna Aphasizheva / Ruslan Aphasizhev / Z Hong Zhou /
PubMed AbstractIn , the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial ...In , the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and gRNA-mRNA-binding RESC-B and RESC-C particles. RESC-A sequesters gRNA termini, thus promoting hairpin formation and blocking mRNA access. The conversion of RESC-A into RESC-B or -C unfolds gRNA and allows mRNA selection. The ensuing gRNA-mRNA duplex protrudes from RESC-B, likely exposing editing sites to RECC-catalyzed cleavage, uridine insertion or deletion, and ligation. Our work reveals a remodeling event facilitating gRNA-mRNA hybridization and assembly of a macromolecular substrate for the editosome's catalytic modality.
External linksScience / PubMed:37410820 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.7 Å
Structure data

29305

8fn4

EMDB-29305, PDB-8fn4:
Cryo-EM structure of RNase-treated RESC-A in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.7 Å

29306

8fn6

EMDB-29306, PDB-8fn6:
Cryo-EM structure of RNase-untreated RESC-A in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.7 Å

29308

8fnc

EMDB-29308, PDB-8fnc:
Cryo-EM structure of RNase-treated RESC-C in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.3 Å

29311

8fnf

EMDB-29311, PDB-8fnf:
Cryo-EM structure of RNase-untreated RESC-C in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.5 Å

29314

8fni

EMDB-29314, PDB-8fni:
Cryo-EM structure of RNase-treated RESC-B in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.4 Å

29316

8fnk

EMDB-29316, PDB-8fnk:
Cryo-EM structure of RNase-untreated RESC-B in trypanosomal RNA editing
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • trypanosoma brucei (eukaryote)
KeywordsRNA BINDING PROTEIN/RNA / HEAT repeat / trypanosoma / RNA editing substrate binding complex / gRNA / RNA BINDING PROTEIN-RNA complex

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