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TitleYeast PIC-Mediator structure with RNA polymerase II C-terminal domain.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 120, Issue 15, Page e2220542120, Year 2023
Publish dateApr 11, 2023
AuthorsSandra Schilbach / Haibo Wang / Christian Dienemann / Patrick Cramer /
PubMed AbstractFor transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas atomic models of the human PIC-Mediator ...For transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas atomic models of the human PIC-Mediator structure have been reported, structures for its yeast counterpart remain incomplete. Here, we present an atomic model for the yeast PIC with core Mediator, including the Mediator middle module that was previously poorly resolved and including subunit Med1 that was previously lacking. We observe three peptide regions containing eleven of the 26 heptapeptide repeats of the flexible C-terminal repeat domain (CTD) of Pol II. Two of these CTD regions bind between the Mediator head and middle modules and form defined CTD-Mediator interactions. CTD peptide 1 binds between the Med6 shoulder and Med31 knob domains, whereas CTD peptide 2 forms additional contacts with Med4. The third CTD region (peptide 3) binds in the Mediator cradle and associates with the Mediator hook. Comparisons with the human PIC-Mediator structure show that the central region in peptide 1 is similar and forms conserved contacts with Mediator, whereas peptides 2 and 3 exhibit distinct structures and Mediator interactions.
External linksProc Natl Acad Sci U S A / PubMed:37014863 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.6 Å
Structure data

16610

8cen

EMDB-16610, PDB-8cen:
Yeast RNA polymerase II transcription pre-initiation complex with core Mediator
Method: EM (single particle) / Resolution: 3.0 Å

16611

8ceo

EMDB-16611, PDB-8ceo:
Yeast RNA polymerase II transcription pre-initiation complex with core Mediator and the +1 nucleosome
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
  • baker's yeast (brewer's yeast)
  • xenopus laevis (African clawed frog)
KeywordsTRANSCRIPTION / PIC / Mediator / TF / nucleosome

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