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TitleStructure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
Journal, issue, pagesEMBO J, Vol. 42, Issue 2, Page e112574, Year 2023
Publish dateDec 12, 2022
AuthorsMd Saiful Islam / Steven W Hardwick / Laura Quell / Svetlana Durica-Mitic / Dimitri Y Chirgadze / Boris Görke / Ben F Luisi /
PubMed AbstractBiogenesis of the essential precursor of the bacterial cell envelope, glucosamine-6-phosphate (GlcN6P), is controlled by intricate post-transcriptional networks mediated by GlmZ, a small regulatory ...Biogenesis of the essential precursor of the bacterial cell envelope, glucosamine-6-phosphate (GlcN6P), is controlled by intricate post-transcriptional networks mediated by GlmZ, a small regulatory RNA (sRNA). GlmZ stimulates translation of the mRNA encoding GlcN6P synthtase in Escherichia coli, but when bound by RapZ protein, the sRNA becomes inactivated through cleavage by the endoribonuclease RNase E. Here, we report the cryoEM structure of the RapZ:GlmZ complex, revealing a complementary match of the RapZ tetrameric quaternary structure to structural repeats in the sRNA. The nucleic acid is contacted by RapZ mostly through a highly conserved domain that shares an evolutionary relationship with phosphofructokinase and suggests links between metabolism and riboregulation. We also present the structure of a precleavage intermediate formed between the binary RapZ:GlmZ complex and RNase E that reveals how GlmZ is presented and recognised by the enzyme. The structures provide a framework for understanding how other encounter complexes might guide recognition and action of endoribonucleases on target transcripts, and how structured substrates in polycistronic precursors may be recognised for processing by RNase E.
External linksEMBO J / PubMed:36504162 / PubMed Central
MethodsEM (single particle)
Resolution3.99 - 4.28 Å
Structure data

15784

8b0i

EMDB-15784, PDB-8b0i:
CryoEM structure of bacterial RapZ.GlmZ complex central to the control of cell envelope biogenesis
Method: EM (single particle) / Resolution: 4.28 Å

15785

8b0j

EMDB-15785, PDB-8b0j:
CryoEM structure of bacterial RNaseE.RapZ.GlmZ complex central to the control of cell envelope biogenesis
Method: EM (single particle) / Resolution: 3.99 Å

Source
  • Escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)
KeywordsRNA BINDING PROTEIN / endonuclease RNase E / adaptor protein RapZ / small regulatory RNA GlmZ

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