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TitleRecognition of the CCT5 di-Glu degron by CRL4 is dependent on TRiC assembly.
Journal, issue, pagesEMBO J, Vol. 42, Issue 4, Page e112253, Year 2023
Publish dateFeb 15, 2023
AuthorsCarlos Pla-Prats / Simone Cavadini / Georg Kempf / Nicolas H Thomä /
PubMed AbstractAssembly Quality Control (AQC) E3 ubiquitin ligases target incomplete or incorrectly assembled protein complexes for degradation. The CUL4-RBX1-DDB1-DCAF12 (CRL4 ) E3 ligase preferentially ...Assembly Quality Control (AQC) E3 ubiquitin ligases target incomplete or incorrectly assembled protein complexes for degradation. The CUL4-RBX1-DDB1-DCAF12 (CRL4 ) E3 ligase preferentially ubiquitinates proteins that carry a C-terminal double glutamate (di-Glu) motif. Reported CRL4 di-Glu-containing substrates include CCT5, a subunit of the TRiC chaperonin. How DCAF12 engages its substrates and the functional relationship between CRL4 and CCT5/TRiC is currently unknown. Here, we present the cryo-EM structure of the DDB1-DCAF12-CCT5 complex at 2.8 Å resolution. DCAF12 serves as a canonical WD40 DCAF substrate receptor and uses a positively charged pocket at the center of the β-propeller to bind the C-terminus of CCT5. DCAF12 specifically reads out the CCT5 di-Glu side chains, and contacts other visible degron amino acids through Van der Waals interactions. The CCT5 C-terminus is inaccessible in an assembled TRiC complex, and functional assays demonstrate that DCAF12 binds and ubiquitinates monomeric CCT5, but not CCT5 assembled into TRiC. Our biochemical and structural results suggest a previously unknown role for the CRL4 E3 ligase in overseeing the assembly of a key cellular complex.
External linksEMBO J / PubMed:36715408 / PubMed Central
MethodsEM (single particle)
Resolution2.83 - 30.6 Å
Structure data

15484

8ajm

EMDB-15484, PDB-8ajm:
Structure of human DDB1-DCAF12 in complex with the C-terminus of CCT5
Method: EM (single particle) / Resolution: 2.83 Å

15485

8ajn

EMDB-15485, PDB-8ajn:
Structure of the human DDB1-DCAF12 complex
Method: EM (single particle) / Resolution: 3.0 Å

15486

8ajo

EMDB-15486, PDB-8ajo:
Negative-stain electron microscopy structure of DDB1-DCAF12-CCT5
Method: EM (single particle) / Resolution: 30.6 Å

Source
  • homo sapiens (human)
KeywordsLIGASE / ubiquitin / E3 / TRiC / chaperonin / chaperone / DDB1 / DCAF12

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