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TitleStructural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II.
Journal, issue, pagesNat Struct Mol Biol, Vol. 29, Issue 12, Page 1159-1169, Year 2022
Publish dateNov 24, 2022
AuthorsSrinivasan Rengachari / Sandra Schilbach / Thangavelu Kaliyappan / Jerome Gouge / Kristina Zumer / Juliane Schwarz / Henning Urlaub / Christian Dienemann / Alessandro Vannini / Patrick Cramer /
PubMed AbstractRNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at ...RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes, such as small nuclear RNA (snRNA) genes, is less well understood at the structural level. Here, we study Pol II initiation at snRNA gene promoters and show that the snRNA-activating protein complex (SNAPc) enables DNA opening and transcription initiation independent of TFIIE and TFIIH in vitro. We then resolve cryo-EM structures of the SNAPc-containing Pol IIpre-initiation complex (PIC) assembled on U1 and U5 snRNA promoters. The core of SNAPc binds two turns of DNA and recognizes the snRNA promoter-specific proximal sequence element (PSE), located upstream of the TATA box-binding protein TBP. Two extensions of SNAPc, called wing-1 and wing-2, bind TFIIA and TFIIB, respectively, explaining how SNAPc directs Pol II to snRNA promoters. Comparison of structures of closed and open promoter complexes elucidates TFIIH-independent DNA opening. These results provide the structural basis of Pol II initiation at non-coding RNA gene promoters.
External linksNat Struct Mol Biol / PubMed:36424526 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.5 Å
Structure data

14996

7zwc

EMDB-14996, PDB-7zwc:
Structure of SNAPc:TBP-TFIIA-TFIIB sub-complex bound to U5 snRNA promoter
Method: EM (single particle) / Resolution: 3.2 Å

14997

7zwd

EMDB-14997, PDB-7zwd:
Structure of SNAPc containing Pol II pre-initiation complex bound to U5 snRNA promoter (CC)
Method: EM (single particle) / Resolution: 3.0 Å

15006

7zx7

EMDB-15006, PDB-7zx7:
Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (CC)
Method: EM (single particle) / Resolution: 3.4 Å

15007

7zx8

EMDB-15007, PDB-7zx8:
Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC)
Method: EM (single particle) / Resolution: 3.0 Å

15009

7zxe

EMDB-15009, PDB-7zxe:
Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC)
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • sus scrofa (pig)
  • pig (pig)
KeywordsTRANSCRIPTION / RNA polymerase II / Pol II / PIC / SNAPc / snRNA / U5 promoter / U1 promoter

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