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TitleMechanism of sphingolipid homeostasis revealed by structural analysis of SPT-ORM1 complex.
Journal, issue, pagesSci Adv, Vol. 9, Issue 13, Page eadg0728, Year 2023
Publish dateMar 29, 2023
AuthorsPeng Liu / Tian Xie / Xinyue Wu / Gongshe Han / Sita D Gupta / Zike Zhang / Jian Yue / Feitong Dong / Kenneth Gable / Somashekarappa Niranjanakumari / Wanyuan Li / Lin Wang / Wenchen Liu / Ruifeng Yao / Edgar B Cahoon / Teresa M Dunn / Xin Gong /
PubMed AbstractThe serine palmitoyltransferase (SPT) complex catalyzes the first and rate-limiting step in sphingolipid biosynthesis in all eukaryotes. ORM/ORMDL proteins are negative regulators of SPT that respond ...The serine palmitoyltransferase (SPT) complex catalyzes the first and rate-limiting step in sphingolipid biosynthesis in all eukaryotes. ORM/ORMDL proteins are negative regulators of SPT that respond to cellular sphingolipid levels. However, the molecular basis underlying ORM/ORMDL-dependent homeostatic regulation of SPT is not well understood. We determined the cryo-electron microscopy structure of SPT-ORM1 complex, composed of LCB1, LCB2a, SPTssa, and ORM1, in an inhibited state. A ceramide molecule is sandwiched between ORM1 and LCB2a in the cytosolic membrane leaflet. Ceramide binding is critical for the ORM1-dependent SPT repression, and dihydroceramides and phytoceramides differentially affect this repression. A hybrid β sheet, formed by the amino termini of ORM1 and LCB2a and induced by ceramide binding, stabilizes the amino terminus of ORM1 in an inhibitory conformation. Our findings provide mechanistic insights into sphingolipid homeostatic regulation via the binding of ceramide to the SPT-ORM/ORMDL complex that may have implications for plant-specific processes such as the hypersensitive response for microbial pathogen resistance.
External linksSci Adv / PubMed:36989369 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.4 Å
Structure data

33873

7yjk

EMDB-33873, PDB-7yjk:
Cryo-EM structure of the dimeric atSPT-ORM1 complex
Method: EM (single particle) / Resolution: 3.2 Å

33874

7yjm

EMDB-33874, PDB-7yjm:
Cryo-EM structure of the monomeric atSPT-ORM1 complex
Method: EM (single particle) / Resolution: 3.2 Å

33875

7yjn

EMDB-33875, PDB-7yjn:
Cryo-EM structure of the monomeric atSPT-ORM1 (ORM1-N17A) complex
Method: EM (single particle) / Resolution: 3.4 Å

33876

7yjo

EMDB-33876, PDB-7yjo:
Cryo-EM structure of the monomeric atSPT-ORM1 (LCB2a-deltaN5) complex
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE / Pyridoxal phosphate

ChemComp-Z1T:
N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide

ChemComp-PLS:
[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSFERASE/INHIBITOR / ceramide / TRANSFERASE-inhibitor complex

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