EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of human Cx36/GJD2 neuronal gap junction channel.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 1347, Year 2023
掲載日	2023年3月11日
著者	Seu-Na Lee / Hwa-Jin Cho / Hyeongseop Jeong / Bumhan Ryu / Hyuk-Joon Lee / Minsoo Kim / Jejoong Yoo / Jae-Sung Woo / Hyung Ho Lee /
PubMed 要旨	Connexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 in normal brain function, the molecular ...Connexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 in normal brain function, the molecular architecture of the Cx36 gap junction channel (GJC) is unknown. Here, we determine cryo-electron microscopy structures of Cx36 GJC at 2.2-3.6 Å resolutions, revealing a dynamic equilibrium between its closed and open states. In the closed state, channel pores are obstructed by lipids, while N-terminal helices (NTHs) are excluded from the pore. In the open state with pore-lining NTHs, the pore is more acidic than those in Cx26 and Cx46/50 GJCs, explaining its strong cation selectivity. The conformational change during channel opening also includes the α-to-π-helix transition of the first transmembrane helix, which weakens the protomer-protomer interaction. Our structural analyses provide high resolution information on the conformational flexibility of Cx36 GJC and suggest a potential role of lipids in the channel gating.
リンク	Nat Commun / PubMed:36906653 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 7.2 Å
構造データ	33254 7xki EMDB-33254, PDB-7xki: Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (D6 symmetry) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-33255: Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (C1 symmetry) 手法: EM (単粒子) / 解像度: 4.5 Å 33256 7xkk EMDB-33256, PDB-7xkk: Human Cx36/GJD2 gap junction channel in detergents 手法: EM (単粒子) / 解像度: 3.2 Å 33270 7xkt EMDB-33270, PDB-7xkt: Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in detergents at 2.2 Angstroms resolution 手法: EM (単粒子) / 解像度: 2.2 Å 33274 7xl8 EMDB-33274, PDB-7xl8: Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (D6 symmetry) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-33275: Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (C1 symmetry) 手法: EM (単粒子) / 解像度: 3.41 Å 33315 7xnh EMDB-33315, PDB-7xnh: Human Cx36/GJD2 gap junction channel with pore-lining N-terminal helices in soybean lipids 手法: EM (単粒子) / 解像度: 3.1 Å 33327 7xnv EMDB-33327, PDB-7xnv: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C6 symmetry) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-33328: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C1 symmetry) 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-34822: Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in soybean lipids 手法: EM (単粒子) / 解像度: 3.4 Å 34856 8hkp EMDB-34856, PDB-8hkp: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C6 symmetry) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-34857: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C1 symmetry) 手法: EM (単粒子) / 解像度: 7.2 Å
化合物	ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / ジパルミトイルホスファチジルグリセロ-ル / リン脂質YM / Phosphatidylglycerol ChemComp-HOH: WATER / 水 ChemComp-AV0: Lauryl Maltose Neopentyl Glycol / ラウリルマルト-スネオペンチルグリコ-ル
由来	homo sapiens (ヒト) escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / connexin 36 (コネクシン) / Gap Junction Channel / Cx36 / GJD2 / BRIL