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TitleCryoEM structures of the multimeric secreted NS1, a major factor for dengue hemorrhagic fever.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 6756, Year 2022
Publish dateNov 9, 2022
AuthorsBo Shu / Justin S G Ooi / Aaron W K Tan / Thiam-Seng Ng / Wanwisa Dejnirattisai / Juthathip Mongkolsapaya / Guntur Fibriansah / Jian Shi / Victor A Kostyuchenko / Gavin R Screaton / Shee-Mei Lok /
PubMed AbstractDengue virus infection can cause dengue hemorrhagic fever (DHF). Dengue NS1 is multifunctional. The intracellular dimeric NS1 (iNS1) forms part of the viral replication complex. Previous studies ...Dengue virus infection can cause dengue hemorrhagic fever (DHF). Dengue NS1 is multifunctional. The intracellular dimeric NS1 (iNS1) forms part of the viral replication complex. Previous studies suggest the extracellular secreted NS1 (sNS1), which is a major factor contributing to DHF, exists as hexamers. The structure of the iNS1 is well-characterised but not that of sNS1. Here we show by cryoEM that the recombinant sNS1 exists in multiple oligomeric states: the tetrameric (stable and loose conformation) and hexameric structures. Stability of the stable and loose tetramers is determined by the conformation of their N-terminal domain - elongated β-sheet or β-roll. Binding of an anti-NS1 Fab breaks the loose tetrameric and hexameric sNS1 into dimers, whereas the stable tetramer remains largely unbound. Our results show detailed quaternary organization of different oligomeric states of sNS1 and will contribute towards the design of dengue therapeutics.
External linksNat Commun / PubMed:36347841 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 8.3 Å
Structure data

32839

7wur

EMDB-32839, PDB-7wur:
CryoEM structure of sNS1 complexed with Fab5E3
Method: EM (single particle) / Resolution: 3.5 Å

32840

7wus

EMDB-32840, PDB-7wus:
CryoEM structure of a dimer of loose sNS1 tetramer
Method: EM (single particle) / Resolution: 3.4 Å

32841

7wut

EMDB-32841, PDB-7wut:
CryoEM structure of stable sNS1 tetramer
Method: EM (single particle) / Resolution: 3.5 Å

32842

7wuu

EMDB-32842, PDB-7wuu:
CryoEM structure of loose sNS1 tetramer
Method: EM (single particle) / Resolution: 8.3 Å

32843

7wuv

EMDB-32843, PDB-7wuv:
CryoEM structure of sNS1 hexamer
Method: EM (single particle) / Resolution: 8.0 Å

Source
  • dengue virus 2
  • homo sapiens (human)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / NS1 / Complex / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex / NS1 protein

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