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TitleCryo-EM structures of human hepatitis B and woodchuck hepatitis virus small spherical subviral particles.
Journal, issue, pagesSci Adv, Vol. 8, Issue 31, Page eabo4184, Year 2022
Publish dateAug 5, 2022
AuthorsHaitao Liu / Xupeng Hong / Ji Xi / Stephan Menne / Jianming Hu / Joseph Che-Yen Wang /
PubMed AbstractThe loss of detectable hepatitis B surface antigen (HBsAg) is considered a functional cure in chronic hepatitis B. Naturally, HBsAg can be incorporated into the virion envelope or assembled into ...The loss of detectable hepatitis B surface antigen (HBsAg) is considered a functional cure in chronic hepatitis B. Naturally, HBsAg can be incorporated into the virion envelope or assembled into subviral particles (SVPs) with lipid from host cells. Until now, there has been no detailed structure of HBsAg, and the published SVP structures are controversial. Here, we report the first subnanometer-resolution structures of spherical SVP from hepatitis B virus (HBV) and the related woodchuck hepatitis virus (WHV) determined by cryo-electron microscopy in combination with AlphaFold2 prediction. Both structures showed unique rhombicuboctahedral symmetry with 24 protruding spikes comprising dimer of small HBsAg with four helical domains. The lipid moiety in the SVP is organized in a noncanonical lipid patch instead of a lipid bilayer, which can accommodate the exposed hydrophobic surface and modulate particle stability. Together, these findings advance our knowledge of viral membrane organization and the structures of HBV and WHV spherical SVPs.
External linksSci Adv / PubMed:35930632 / PubMed Central
MethodsEM (single particle)
Resolution6.3 - 9.7 Å
Structure data

26117

7tuk

EMDB-26117: Hepatitis B virus small spherical subviral particle
PDB-7tuk: Small hepatitis B virus surface protein without cytosolic and antigenic loops
Method: EM (single particle) / Resolution: 6.3 Å

26118

7tul

EMDB-26118: Woodchuck hepatitis virus small spherical subviral particle
PDB-7tul: Woodchuck hepatitis small surface protein without cytosolic and antigenic loops
Method: EM (single particle) / Resolution: 6.5 Å

EMDB-26119: Woodchuck hepatitis B virus small spherical subviral particle treated with 0.1% NP-40
Method: EM (single particle) / Resolution: 9.7 Å

Source
  • hbv genotype e (virus)
  • woodchuck hepatitis virus
KeywordsVIRAL PROTEIN / HBV / SVP / HBsAg / Envelope protein / WHV / WHsAg

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