EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of ATP synthase under strain during catalysis.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 2232, Year 2022
掲載日	2022年4月25日
著者	Hui Guo / John L Rubinstein /
PubMed 要旨	ATP synthases are macromolecular machines consisting of an ATP-hydrolysis-driven F motor and a proton-translocation-driven F motor. The F and F motors oppose each other's action on a shared rotor ...ATP synthases are macromolecular machines consisting of an ATP-hydrolysis-driven F motor and a proton-translocation-driven F motor. The F and F motors oppose each other's action on a shared rotor subcomplex and are held stationary relative to each other by a peripheral stalk. Structures of resting mitochondrial ATP synthases revealed a left-handed curvature of the peripheral stalk even though rotation of the rotor, driven by either ATP hydrolysis in F or proton translocation through F, would apply a right-handed bending force to the stalk. We used cryoEM to image yeast mitochondrial ATP synthase under strain during ATP-hydrolysis-driven rotary catalysis, revealing a large deformation of the peripheral stalk. The structures show how the peripheral stalk opposes the bending force and suggests that during ATP synthesis proton translocation causes accumulation of strain in the stalk, which relaxes by driving the relative rotation of the rotor through six sub-steps within F, leading to catalysis.
リンク	Nat Commun / PubMed:35468906 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 8.4 Å
構造データ	25930 7tjs EMDB-25930, PDB-7tjs: Yeast ATP synthase F1 region State 1-3catalytic beta_tight closed without exogenous ATP 手法: EM (単粒子) / 解像度: 3.2 Å 25931 7tjt EMDB-25931, PDB-7tjt: Yeast ATP synthase F1 region State 1-3catalytic beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.2 Å 25932 7tju EMDB-25932, PDB-7tju: Yeast ATP synthase F1 region State 1-3binding beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.3 Å 25933 7tjv EMDB-25933, PDB-7tjv: Yeast ATP synthase F1 region State 1catalytic(a) with 10 mM ATP 手法: EM (単粒子) / 解像度: 3.6 Å 25934 7tjw EMDB-25934, PDB-7tjw: Yeast ATP synthase F1 region State 1catalytic(e-h) with 10 mM ATP 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-25935: Yeast ATP synthase F1 region State 1catalytic beta_tight closed without exogenous ATP 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-25936: Yeast ATP synthase F1 region State 1catalytic beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-25937: Yeast ATP synthase F1 region State 1binding beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25938: Yeast ATP synthase F1 region State 2catalytic beta_tight closed without exogenous ATP 手法: EM (単粒子) / 解像度: 3.4 Å 25939 7tjx EMDB-25939, PDB-7tjx: Yeast ATP synthase F1 region State 1binding(a-d) with 10 mM ATP 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-25940: Yeast ATP synthase F1 region State 2catalytic beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-25941: Yeast ATP synthase F1 region State 2binding beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-25942: Yeast ATP synthase F1 region State 3catalytic beta_tight closed without exogenous ATP 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-25943: Yeast ATP synthase F1 region State 3catalytic beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-25944: Yeast ATP synthase F1 region State 3binding beta_tight open without exogenous ATP 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25945: Yeast ATP synthase OSCP without exogenous ATP 手法: EM (単粒子) / 解像度: 3.3 Å 25946 7tjy EMDB-25946, PDB-7tjy: Yeast ATP synthase State 1catalytic(a) without exogenous ATP backbone model 手法: EM (単粒子) / 解像度: 3.8 Å 25947 7tjz EMDB-25947, PDB-7tjz: Yeast ATP synthase State 1catalytic(b) without exogenous ATP backbone model 手法: EM (単粒子) / 解像度: 4.4 Å 25948 7tk0 EMDB-25948, PDB-7tk0: Yeast ATP synthase State 1catalytic(c) without exogenous ATP backbone model 手法: EM (単粒子) / 解像度: 4.4 Å 25949 7tk1 EMDB-25949, PDB-7tk1: Yeast ATP synthase State 1catalytic(d) without exogenous ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-25950: Yeast ATP synthase peripheral stalk without exogenous ATP 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25951: Yeast ATP synthase FO region without exogenous ATP 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25952: Yeast ATP synthase composite map without exogenous ATP 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-25953: Yeast ATP synthase with 10 mM ATP showing strained peripheral stalk determined by a screening microscope 手法: EM (単粒子) / 解像度: 8.4 Å 25954 7tk2 EMDB-25954, PDB-7tk2: Yeast ATP synthase State 1binding(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.5 Å 25955 7tk3 EMDB-25955, PDB-7tk3: Yeast ATP synthase State 1binding(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.3 Å 25956 7tk4 EMDB-25956, PDB-7tk4: Yeast ATP synthase State 1binding(c) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.0 Å 25957 7tk5 EMDB-25957, PDB-7tk5: Yeast ATP synthase State 1binding(d) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.8 Å 25958 7tk6 EMDB-25958, PDB-7tk6: Yeast ATP synthase State 1catalytic(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.5 Å 25959 7tk7 EMDB-25959, PDB-7tk7: Yeast ATP synthase State 1catalytic(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.7 Å 25960 7tk8 EMDB-25960, PDB-7tk8: Yeast ATP synthase State 1catalytic(c) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.7 Å 25961 7tk9 EMDB-25961, PDB-7tk9: Yeast ATP synthase State 1catalytic(d) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.0 Å 25962 7tka EMDB-25962, PDB-7tka: Yeast ATP synthase State 1catalytic(e) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å 25963 7tkb EMDB-25963, PDB-7tkb: Yeast ATP synthase State 1catalytic(f) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.3 Å 25964 7tkc EMDB-25964, PDB-7tkc: Yeast ATP synthase State 1catalytic(g) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 5.8 Å 25965 7tkd EMDB-25965, PDB-7tkd: Yeast ATP synthase State 1catalytic(h) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.7 Å 25966 7tke EMDB-25966, PDB-7tke: Yeast ATP synthase State 2binding(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å 25967 7tkf EMDB-25967, PDB-7tkf: Yeast ATP synthase State 2binding(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å 25968 7tkg EMDB-25968, PDB-7tkg: Yeast ATP synthase State 2catalytic(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.5 Å 25969 7tkh EMDB-25969, PDB-7tkh: Yeast ATP synthase State 2catalytic(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.4 Å 25970 7tki EMDB-25970, PDB-7tki: Yeast ATP synthase State 2catalytic(c) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å 25971 7tkj EMDB-25971, PDB-7tkj: Yeast ATP synthase State 2catalytic(d) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.5 Å 25972 7tkk EMDB-25972, PDB-7tkk: Yeast ATP synthase State 2catalytic(e) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.3 Å 25973 7tkl EMDB-25973, PDB-7tkl: Yeast ATP synthase State 3binding(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.4 Å 25974 7tkm EMDB-25974, PDB-7tkm: Yeast ATP synthase State 3binding(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.5 Å 25975 7tkn EMDB-25975, PDB-7tkn: Yeast ATP synthase State 3binding(c) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.1 Å 25976 7tko EMDB-25976, PDB-7tko: Yeast ATP synthase State 3catalytic(a) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.8 Å 25977 7tkp EMDB-25977, PDB-7tkp: Yeast ATP synthase State 3catalytic(b) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.6 Å 25978 7tkq EMDB-25978, PDB-7tkq: Yeast ATP synthase State 3catalytic(c) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 4.5 Å 25979 7tkr EMDB-25979, PDB-7tkr: Yeast ATP synthase State 3catalytic(d) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 6.5 Å 25980 7tks EMDB-25980, PDB-7tks: Yeast ATP synthase State 3catalytic(e) with 10 mM ATP backbone model 手法: EM (単粒子) / 解像度: 7.5 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩
由来	saccharomyces cerevisiae (パン酵母)
キーワード	HYDROLASE (加水分解酵素) / F1-ATPase / ATP Synthase (ATP合成酵素) / Nanomotor / Complex