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TitlePlant phytochrome B is an asymmetric dimer with unique signalling potential.
Journal, issue, pagesNature, Vol. 604, Issue 7904, Page 127-133, Year 2022
Publish dateMar 30, 2022
AuthorsHua Li / E Sethe Burgie / Zira T K Gannam / Huilin Li / Richard D Vierstra /
PubMed AbstractMany aspects of plant photoperception are mediated by the phytochrome (Phy) family of bilin-containing photoreceptors that reversibly interconvert between inactive Pr and active Pfr conformers. ...Many aspects of plant photoperception are mediated by the phytochrome (Phy) family of bilin-containing photoreceptors that reversibly interconvert between inactive Pr and active Pfr conformers. Despite extensive biochemical studies, full understanding of plant Phy signalling has remained unclear due to the absence of relevant 3D models. Here we report a cryo-electron microscopy structure of Arabidopsis PhyB in the Pr state that reveals a topologically complex dimeric organization that is substantially distinct from its prokaryotic relatives. Instead of an anticipated parallel architecture, the C-terminal histidine-kinase-related domains (HKRDs) associate head-to-head, whereas the N-terminal photosensory regions associate head-to-tail to form a parallelogram-shaped platform with near two-fold symmetry. The platform is internally linked by the second of two internal Per/Arnt/Sim domains that binds to the photosensory module of the opposing protomer and a preceding 'modulator' loop that assembles tightly with the photosensory module of its own protomer. Both connections accelerate the thermal reversion of Pfr back to Pr, consistent with an inverse relationship between dimer assembly and Pfr stability. Lopsided contacts between the HKRDs and the platform create profound asymmetry to PhyB that might imbue distinct signalling potentials to the protomers. We propose that this unique structural dynamism creates an extensive photostate-sensitive surface for conformation-dependent interactions between plant Phy photoreceptors and their signalling partners.
External linksNature / PubMed:35355010 / PubMed Central
MethodsEM (single particle)
Resolution3.3 Å
Structure data

24780

7rzw

EMDB-24780: CryoEM map of Arabidopsis thaliana phytochrome B
PDB-7rzw: CryoEM structure of Arabidopsis thaliana phytochrome B
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-O6E:
3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid

Source
  • arabidopsis thaliana (thale cress)
KeywordsGENE REGULATION / phytochrome / asymmetric dimer

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