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Structure paper

TitleSimultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling.
Journal, issue, pagesCell, Vol. 184, Issue 8, Page 2103-2120.e31, Year 2021
Publish dateApr 15, 2021
AuthorsRoss A Robinson / Samuel C Griffiths / Lieke L van de Haar / Tomas Malinauskas / Eljo Y van Battum / Pavol Zelina / Rebekka A Schwab / Dimple Karia / Lina Malinauskaite / Sara Brignani / Marleen H van den Munkhof / Özge Düdükcü / Anna A De Ruiter / Dianne M A Van den Heuvel / Benjamin Bishop / Jonathan Elegheert / A Radu Aricescu / R Jeroen Pasterkamp / Christian Siebold /
PubMed AbstractDuring cell migration or differentiation, cell surface receptors are simultaneously exposed to different ligands. However, it is often unclear how these extracellular signals are integrated. Neogenin ...During cell migration or differentiation, cell surface receptors are simultaneously exposed to different ligands. However, it is often unclear how these extracellular signals are integrated. Neogenin (NEO1) acts as an attractive guidance receptor when the Netrin-1 (NET1) ligand binds, but it mediates repulsion via repulsive guidance molecule (RGM) ligands. Here, we show that signal integration occurs through the formation of a ternary NEO1-NET1-RGM complex, which triggers reciprocal silencing of downstream signaling. Our NEO1-NET1-RGM structures reveal a "trimer-of-trimers" super-assembly, which exists in the cell membrane. Super-assembly formation results in inhibition of RGMA-NEO1-mediated growth cone collapse and RGMA- or NET1-NEO1-mediated neuron migration, by preventing formation of signaling-compatible RGM-NEO1 complexes and NET1-induced NEO1 ectodomain clustering. These results illustrate how simultaneous binding of ligands with opposing functions, to a single receptor, does not lead to competition for binding, but to formation of a super-complex that diminishes their functional outputs.
External linksCell / PubMed:33740419 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.15 - 5.98 Å
Structure data

12286

7ndg

EMDB-12286, PDB-7ndg:
Cryo-EM structure of the ternary complex between Netrin-1, Neogenin and Repulsive Guidance Molecule B
Method: EM (single particle) / Resolution: 5.98 Å

PDB-7ne0:
Structure of the ternary complex between Netrin-1, Repulsive-Guidance Molecule-B (RGMB) and Neogenin
Method: X-RAY DIFFRACTION / Resolution: 3.25 Å

PDB-7ne1:
Structure of the complex between Netrin-1 and its receptor Neogenin
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-NO3:
NITRATE ION / Nitrate

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsSIGNALING PROTEIN / Signal transduction / cell surface receptors / neuron regeneration / cell migration / Netrin / Neogenin / Repulsive Guidance Molecule / complex structure / protein-protein interactions / cell surface receptor signaling / axon guidance / migration / cancer / growth cone / receptor clustering

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