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TitleStructural basis for the constitutive activity and immunomodulatory properties of the Epstein-Barr virus-encoded G protein-coupled receptor BILF1.
Journal, issue, pagesImmunity, Vol. 54, Issue 7, Page 1405-11416.e7, Year 2021
Publish dateJul 13, 2021
AuthorsNaotaka Tsutsumi / Qianhui Qu / Maša Mavri / Maibritt S Baggesen / Shoji Maeda / Deepa Waghray / Christian Berg / Brian K Kobilka / Mette M Rosenkilde / Georgios Skiniotis / K Christopher Garcia /
PubMed AbstractEpstein-Barr virus (EBV) encodes a G protein-coupled receptor (GPCR) termed BILF1 that is essential for EBV-mediated immunosuppression and oncogenesis. BILF1 couples with inhibitory G protein (Gi), ...Epstein-Barr virus (EBV) encodes a G protein-coupled receptor (GPCR) termed BILF1 that is essential for EBV-mediated immunosuppression and oncogenesis. BILF1 couples with inhibitory G protein (Gi), the major intracellular signaling effector for human chemokine receptors, and exhibits constitutive signaling activity; the ligand(s) for BILF1 are unknown. We studied the origins of BILF1's constitutive activity through structure determination of BILF1 bound to the inhibitory G protein (Gi) heterotrimer. The 3.2-Å resolution cryo-electron microscopy structure revealed an extracellular loop within BILF1 that blocked the typical chemokine binding site, suggesting ligand-autonomous receptor activation. Rather, amino acid substitutions within BILF1 transmembrane regions at hallmark ligand-activated class A GPCR "microswitches" stabilized a constitutively active BILF1 conformation for Gi coupling in a ligand-independent fashion. Thus, the constitutive activity of BILF1 promotes immunosuppression and virulence independent of ligand availability, with implications for the function of GPCRs encoded by related viruses and for therapeutic targeting of EBV.
External linksImmunity / PubMed:34216564 / PubMed Central
MethodsEM (single particle)
Resolution3.2 Å
Structure data

22338

7jhj

EMDB-22338, PDB-7jhj:
Structure of the Epstein-Barr virus GPCR BILF1 in complex with human Gi
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • Human gammaherpesvirus 4 (Epstein-Barr virus)
  • epstein-barr virus (Epstein-Barr virus)
KeywordsMEMBRANE PROTEIN / viral GPCR / class A-like GPCR / Epstein-Barr virus

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