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-Structure paper
Title | Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60. |
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Journal, issue, pages | Chem Commun (Camb), Vol. 57, Issue 79, Page 10226-10229, Year 2021 |
Publish date | Oct 5, 2021 |
Authors | Junya Obata / Norifumi Kawakami / Akihisa Tsutsumi / Erika Nasu / Kenji Miyamoto / Masahide Kikkawa / Ryoichi Arai / |
PubMed Abstract | Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self- ...Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications. |
External links | Chem Commun (Camb) / PubMed:34523636 |
Methods | EM (single particle) |
Resolution | 3.3 Å |
Structure data | EMDB-31256, PDB-7eq9: |
Source |
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Keywords | DE NOVO PROTEIN / Artificial designed protein complex / VIRUS LIKE PARTICLE |