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TitleSubstrate-engaged type III secretion system structures reveal gating mechanism for unfolded protein translocation.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 1546, Year 2021
Publish dateMar 9, 2021
AuthorsSean Miletic / Dirk Fahrenkamp / Nikolaus Goessweiner-Mohr / Jiri Wald / Maurice Pantel / Oliver Vesper / Vadim Kotov / Thomas C Marlovits /
PubMed AbstractMany bacterial pathogens rely on virulent type III secretion systems (T3SSs) or injectisomes to translocate effector proteins in order to establish infection. The central component of the injectisome ...Many bacterial pathogens rely on virulent type III secretion systems (T3SSs) or injectisomes to translocate effector proteins in order to establish infection. The central component of the injectisome is the needle complex which assembles a continuous conduit crossing the bacterial envelope and the host cell membrane to mediate effector protein translocation. However, the molecular principles underlying type III secretion remain elusive. Here, we report a structure of an active Salmonella enterica serovar Typhimurium needle complex engaged with the effector protein SptP in two functional states, revealing the complete 800Å-long secretion conduit and unraveling the critical role of the export apparatus (EA) subcomplex in type III secretion. Unfolded substrates enter the EA through a hydrophilic constriction formed by SpaQ proteins, which enables side chain-independent substrate transport. Above, a methionine gasket formed by SpaP proteins functions as a gate that dilates to accommodate substrates while preventing leaky pore formation. Following gate penetration, a moveable SpaR loop first folds up to then support substrate transport. Together, these findings establish the molecular basis for substrate translocation through T3SSs and improve our understanding of bacterial pathogenicity and motility.
External linksNat Commun / PubMed:33750771 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.6 Å
Structure data

11780

7agx

EMDB-11780, PDB-7agx:
Apo-state type 3 secretion system export apparatus complex from Salmonella enterica typhimurium
Method: EM (single particle) / Resolution: 3.6 Å

11781

7ah9

7ahi

EMDB-11781, PDB-7ah9:
Substrate-engaged type 3 secretion system needle complex from Salmonella enterica typhimurium - SpaR state 1
PDB-7ahi: Substrate-engaged type 3 secretion system needle complex from Salmonella enterica typhimurium - SpaR state 2
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / Phosphatidic acid

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM / Lauryldimethylamine oxide

Source
  • salmonella enterica subsp. enterica serovar typhimurium str. lt2 (bacteria)
  • salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720) (bacteria)
KeywordsPROTEIN TRANSPORT / T3SS / Export Apparatus / Injectisome / Needle Complex / Substrate

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