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TitleThe Halastavi árva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism.
Journal, issue, pagesCell Rep, Vol. 33, Issue 10, Page 108476, Year 2020
Publish dateDec 8, 2020
AuthorsIrina S Abaeva / Quentin Vicens / Anthony Bochler / Heddy Soufari / Angelita Simonetti / Tatyana V Pestova / Yaser Hashem / Christopher U T Hellen /
PubMed AbstractDicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the ...Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the elongation cycle via formation of IRES/80S complexes resembling the pre-translocation state. eEF2 then translocates the [codon-anticodon]-mimicking pseudoknot I (PKI) from ribosomal A sites to P sites, bringing the first sense codon into the decoding center. Halastavi árva virus (HalV) contains an IGR that is related to previously described IGR IRESs but lacks domain 2, which enables these IRESs to bind to individual 40S ribosomal subunits. By using in vitro reconstitution and cryoelectron microscopy (cryo-EM), we now report that the HalV IGR IRES functions by the simplest initiation mechanism that involves binding to 80S ribosomes such that PKI is placed in the P site, so that the A site contains the first codon that is directly accessible for decoding without prior eEF2-mediated translocation of PKI.
External linksCell Rep / PubMed:33296660 / PubMed Central
MethodsEM (single particle)
Resolution3.49 - 3.6 Å
Structure data

11459

6zvk

EMDB-11459, PDB-6zvk:
The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism
Method: EM (single particle) / Resolution: 3.49 Å

11590

7a01

EMDB-11590, PDB-7a01:
The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • halastavi arva rna virus
  • Rabbit (rabbit)
KeywordsTRANSLATION / RIBOSOME INTERNAL RIBOSOME ENTRY SITE RNA DICISTROVIRUS Halastavi arva virus Intergenic region pseudoknot CrPV IGR IRES SERBP1 / RIBOSOME / Dicistrovirus Halastavi arva virus Intergenic region Internal ribosome entry site IRES RNA pseudoknot ribosome SERBP1 CrPV IGR IRES

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