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TitleStructural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis.
Journal, issue, pagesMol Cell, Vol. 79, Issue 4, Page 629-644.e4, Year 2020
Publish dateAug 20, 2020
AuthorsMateusz Jaskolowski / David J F Ramrath / Philipp Bieri / Moritz Niemann / Simone Mattei / Salvatore Calderaro / Marc Leibundgut / Elke K Horn / Daniel Boehringer / André Schneider / Nenad Ban /
PubMed AbstractIn contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence ...In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation.
External linksMol Cell / PubMed:32679035
MethodsEM (single particle)
Resolution3.1 - 3.9 Å
Structure data

10999

6yxx

EMDB-10999, PDB-6yxx:
State A of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Method: EM (single particle) / Resolution: 3.9 Å

11000

6yxy

EMDB-11000, PDB-6yxy:
State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-NA:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

ChemComp-HOH:
WATER / Water

ChemComp-SPD:
SPERMIDINE / Spermidine

Source
  • trypanosoma brucei brucei (eukaryote)
KeywordsRIBOSOME / mitoribosome / assembly / LSU

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