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TitleStructural and biochemical characterization of in vivo assembled Lactococcus lactis CRISPR-Csm complex.
Journal, issue, pagesCommun Biol, Vol. 5, Issue 1, Page 279, Year 2022
Publish dateMar 29, 2022
AuthorsSagar Sridhara / Jay Rai / Charlisa Whyms / Hemant Goswami / Huan He / Walter Woodside / Michael P Terns / Hong Li /
PubMed AbstractThe small RNA-mediated immunity in bacteria depends on foreign RNA-activated and self RNA-inhibited enzymatic activities. The multi-subunit Type III-A CRISPR-Cas effector complex (Csm) exemplifies ...The small RNA-mediated immunity in bacteria depends on foreign RNA-activated and self RNA-inhibited enzymatic activities. The multi-subunit Type III-A CRISPR-Cas effector complex (Csm) exemplifies this principle and is in addition regulated by cellular metabolites such as divalent metals and ATP. Recognition of the foreign or cognate target RNA (CTR) triggers its single-stranded deoxyribonuclease (DNase) and cyclic oligoadenylate (cOA) synthesis activities. The same activities remain dormant in the presence of the self or non-cognate target RNA (NTR) that differs from CTR only in its 3'-protospacer flanking sequence (3'-PFS). Here we employ electron cryomicroscopy (cryoEM), functional assays, and comparative cross-linking to study in vivo assembled mesophilic Lactococcus lactis Csm (LlCsm) at the three functional states: apo, the CTR- and the NTR-bound. Unlike previously studied Csm complexes, we observed binding of 3'-PFS to Csm in absence of bound ATP and analyzed the structures of the four RNA cleavage sites. Interestingly, comparative crosslinking results indicate a tightening of the Csm3-Csm4 interface as a result of CTR but not NTR binding, reflecting a possible role of protein dynamics change during activation.
External linksCommun Biol / PubMed:35351985 / PubMed Central
MethodsEM (single particle)
Resolution2.97 - 3.47 Å
Structure data

22266

6xn3

EMDB-22266, PDB-6xn3:
Structure of the Lactococcus lactis Csm CTR_4:3 CRISPR-Cas Complex
Method: EM (single particle) / Resolution: 3.0 Å

22267

6xn4

EMDB-22267, PDB-6xn4:
Structure of the Lactococcus lactis Csm CTR_3:2 CRISPR-Cas Complex
Method: EM (single particle) / Resolution: 3.35 Å

22268

6xn5

EMDB-22268, PDB-6xn5:
Structure of the Lactococcus lactis Csm Apo- CRISPR-Cas Complex
Method: EM (single particle) / Resolution: 2.97 Å

22269

6xn7

EMDB-22269, PDB-6xn7:
Structure of the Lactococcus lactis Csm NTR CRISPR-Cas Complex
Method: EM (single particle) / Resolution: 3.47 Å

Source
  • lactococcus lactis subsp. lactis (lactic acid bacteria)
KeywordsRNA BINDING PROTEIN/RNA / Type III-A CRISPR-Cas / Csm / RNA BINDING PROTEIN-RNA complex

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