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TitleThe structures of natively assembled clathrin-coated vesicles.
Journal, issue, pagesSci Adv, Vol. 6, Issue 30, Page eaba8397, Year 2020
Publish dateJul 22, 2020
AuthorsMohammadreza Paraan / Joshua Mendez / Savanna Sharum / Danielle Kurtin / Huan He / Scott M Stagg /
PubMed AbstractClathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy ...Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
External linksSci Adv / PubMed:32743076 / PubMed Central
MethodsEM (single particle)
Resolution6.3 - 17.9 Å
Structure data

EMDB-21608:
Mini-coat geometry for a clathrin coated vesicle: clathrin-focused
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-21609:
Mini-coat geometry for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 12.0 Å

EMDB-21610:
Basket geometry for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 15.6 Å

21611

6wcj

EMDB-21611: Asymmetric vertex of the clathrin minicoat cage, subparticle refinement of the clathrin layer
PDB-6wcj: Asymmetric vertex of the clathrin minicoat cage
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-21612:
Football geometry for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 15.1 Å

EMDB-21613:
Tennis geometry for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 17.2 Å

EMDB-21614:
Barrel geometry for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 17.9 Å

EMDB-21615:
Pentagonal face of the mini-coat cage for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 8.3 Å

EMDB-21616:
Hexagonal face of the mini-coat cage for a clathrin coated vesicle
Method: EM (single particle) / Resolution: 9.1 Å

Source
  • bos taurus (cattle)
  • bovine (cattle)
KeywordsENDOCYTOSIS / Clathrin coated vesicle / clathrin heavy chain / clathrin light chain / clathrin cage

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