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| Title | Distortion of the bilayer and dynamics of the BAM complex in lipid nanodiscs. |
|---|---|
| Journal, issue, pages | Commun Biol, Vol. 3, Issue 1, Page 766, Year 2020 |
| Publish date | Dec 14, 2020 |
 Authors | Matthew G Iadanza / Bob Schiffrin / Paul White / Matthew A Watson / Jim E Horne / Anna J Higgins / Antonio N Calabrese / David J Brockwell / Roman Tuma / Antreas C Kalli / Sheena E Radford / Neil A Ranson /   |
| PubMed Abstract | The β-barrel assembly machinery (BAM) catalyses the folding and insertion of β-barrel outer membrane proteins (OMPs) into the outer membranes of Gram-negative bacteria by mechanisms that remain ...The β-barrel assembly machinery (BAM) catalyses the folding and insertion of β-barrel outer membrane proteins (OMPs) into the outer membranes of Gram-negative bacteria by mechanisms that remain unclear. Here, we present an ensemble of cryoEM structures of the E. coli BamABCDE (BAM) complex in lipid nanodiscs, determined using multi-body refinement techniques. These structures, supported by single-molecule FRET measurements, describe a range of motions in the BAM complex, mostly localised within the periplasmic region of the major subunit BamA. The β-barrel domain of BamA is in a 'lateral open' conformation in all of the determined structures, suggesting that this is the most energetically favourable species in this bilayer. Strikingly, the BAM-containing lipid nanodisc is deformed, especially around BAM's lateral gate. This distortion is also captured in molecular dynamics simulations, and provides direct structural evidence for the lipid 'disruptase' activity of BAM, suggested to be an important part of its functional mechanism. |
 External links | Commun Biol / PubMed:33318620 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 6.65 - 10.8 Å |
| Structure data | EMDB-10247, PDB-6smx: EMDB-10248, PDB-6sn0: EMDB-10249, PDB-6sn2: EMDB-10250, PDB-6sn3: EMDB-10251, PDB-6sn4: EMDB-10252, PDB-6sn5: EMDB-10253, PDB-6sn7: EMDB-10254, PDB-6sn8: EMDB-10255, PDB-6sn9: EMDB-10268, PDB-6so7: EMDB-10269, PDB-6so8: EMDB-10270, PDB-6soa: EMDB-10271, PDB-6sob: EMDB-10272, PDB-6soc: EMDB-10274, PDB-6sog: EMDB-10275, PDB-6soh: EMDB-10276, PDB-6soj: |
| Source |
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 Keywords |  MEMBRANE PROTEIN / Outer membrane / OMP / beta-barrel / folding / insertion |
