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TitleAn open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7.
Journal, issue, pagesRNA, Vol. 27, Issue 2, Page 221-233, Year 2021
Publish dateNov 20, 2020
AuthorsJay Rai / Melissa D Parker / Haina Huang / Stefan Choy / Homa Ghalei / Matthew C Johnson / Katrin Karbstein / M Elizabeth Stroupe /
PubMed AbstractDuring their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly ...During their maturation, nascent 40S subunits enter a translation-like quality control cycle, where they are joined by mature 60S subunits to form 80S-like ribosomes. While these assembly intermediates are essential for maturation and quality control, how they form, and how their structure promotes quality control, remains unknown. To address these questions, we determined the structure of an 80S-like ribosome assembly intermediate to an overall resolution of 3.4 Å. The structure, validated by biochemical data, resolves a large body of previously paradoxical data and illustrates how assembly and translation factors cooperate to promote the formation of an interface that lacks many mature subunit contacts but is stabilized by the universally conserved methyltransferase Dim1. We also show how Tsr1 enables this interface by blocking the canonical binding of eIF5B to 40S subunits, while maintaining its binding to 60S. The structure also shows how this interface leads to unfolding of the platform, which allows for temporal regulation of the ATPase Fap7, thus linking 40S maturation to quality control during ribosome assembly.
External linksRNA / PubMed:33219089 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.8 Å
Structure data

20077

6oig

EMDB-20077, PDB-6oig:
Subunit joining exposes nascent pre-40S rRNA for processing and quality control
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-20211:
Subunit joining exposes nascent pre-40S rRNA for processing and quality control
Method: EM (single particle) / Resolution: 3.4 Å

21644

6wdr

EMDB-21644, PDB-6wdr:
Subunit joining exposes nascent pre-40S rRNA for processing and quality control
Method: EM (single particle) / Resolution: 3.7 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsRIBOSOME / 80S-like complex / 60S / 80S-like / Tsr1 / Structural heterogeneity

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