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Structure paper

TitleStructure of the gene therapy vector, adeno-associated virus with its cell receptor, AAVR.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateMay 22, 2019
AuthorsNancy L Meyer / Guiqing Hu / Omar Davulcu / Qing Xie / Alex J Noble / Craig Yoshioka / Drew S Gingerich / Andrew Trzynka / Larry David / Scott M Stagg / Michael Stewart Chapman /
PubMed AbstractAdeno-associated virus (AAV) vectors are preeminent in emerging clinical gene therapies. Generalizing beyond the most tractable genetic diseases will require modulation of cell specificity and immune ...Adeno-associated virus (AAV) vectors are preeminent in emerging clinical gene therapies. Generalizing beyond the most tractable genetic diseases will require modulation of cell specificity and immune neutralization. Interactions of AAV with its cellular receptor, AAVR, are key to understanding cell-entry and trafficking with the rigor needed to engineer tissue-specific vectors. -electron tomography shows ordered binding of part of the flexible receptor to the viral surface, with distal domains in multiple conformations. Regions of the virus and receptor in close physical proximity can be identified by cross-linking/mass spectrometry. -electron microscopy with a two-domain receptor fragment reveals the interactions at 2.4 Å resolution. AAVR binds between AAV's spikes on a plateau that is conserved, except in one clade whose structure is AAVR-incompatible. AAVR's footprint overlaps the epitopes of several neutralizing antibodies, prompting a re-evaluation of neutralization mechanisms. The structure provides a roadmap for experimental probing and manipulation of viral-receptor interactions.
External linksElife / PubMed:31115336 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging) / EM (tomography)
Resolution2.4 - 20.0 Å
Structure data

0553

6nz0

EMDB-0553, PDB-6nz0:
Cryo-EM structure of AAV-2 in complex with AAVR PKD domains 1 and 2
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-0621:
Structure of the AAV2 with its Cell Receptor, AAVR
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

EMDB-0622:
Structure of the AAV2 with its Cell Receptor, AAVR
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

EMDB-0623:
Structure of the AAV2 with its Cell Receptor, AAVR
Method: EM (tomography) / Resolution: 20.0 Å

EMDB-0624:
Structure of the AAV2 with its Cell Receptor, AAVR
Method: EM (tomography) / Resolution: 10.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • adeno-associated virus - 2
  • Adeno-associated virus
KeywordsVIRUS / AAV / AAVR / receptor / coreceptor / KIAA0319L / PKD / parvovirus

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