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TitleAmorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation.
Journal, issue, pagesProg Biophys Mol Biol, Vol. 156, Page 3-13, Year 2020
Publish dateAug 3, 2020
AuthorsXiaojun Huang / Lei Zhang / Zuoling Wen / Hui Chen / Shuoguo Li / Gang Ji / Chang-Cheng Yin / Fei Sun /
PubMed AbstractCryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and ...Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, data collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation as a layer of holey supporting film covering on TEM grid. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including much better electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM data collection procedure.
External linksProg Biophys Mol Biol / PubMed:32758492
MethodsEM (single particle)
Resolution2.4 - 2.6 Å
Structure data

30083

6m52

EMDB-30083: Human apo ferritin chain A frozen on TEM grid with amorphous carbon supporting film
PDB-6m52: Human apo ferritin frozen on TEM grid with amorphous carbon supporting film
Method: EM (single particle) / Resolution: 2.6 Å

30084

6m54

EMDB-30084, PDB-6m54:
Human apo ferritin frozen on TEM grid with Amorphous nickel titanium alloy supporting film
Method: EM (single particle) / Resolution: 2.4 Å

Chemicals

ChemComp-FE2:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
KeywordsOXIDOREDUCTASE / Apoferritin heavy chain / Homo sapiens / METAL TRANSPORT

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