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TitleCapsid protein structure in Zika virus reveals the flavivirus assembly process.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 895, Year 2020
Publish dateFeb 14, 2020
AuthorsTer Yong Tan / Guntur Fibriansah / Victor A Kostyuchenko / Thiam-Seng Ng / Xin-Xiang Lim / Shuijun Zhang / Xin-Ni Lim / Jiaqi Wang / Jian Shi / Marc C Morais / Davide Corti / Shee-Mei Lok /
PubMed AbstractStructures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution and show detailed structure and arrangement of their surface proteins (E and prM in immature virus ...Structures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution and show detailed structure and arrangement of their surface proteins (E and prM in immature virus or M in mature virus). By contrast, the arrangement of the capsid proteins:RNA complex, which forms the core of the particle, is poorly understood, likely due to inherent dynamics. Here, we stabilize immature Zika virus via an antibody that binds across the E and prM proteins, resulting in a subnanometer resolution structure of capsid proteins within the virus particle. Fitting of the capsid protein into densities shows the presence of a helix previously thought to be removed via proteolysis. This structure illuminates capsid protein quaternary organization, including its orientation relative to the lipid membrane and the genomic RNA, and its interactions with the transmembrane regions of the surface proteins. Results show the capsid protein plays a central role in the flavivirus assembly process.
External linksNat Commun / PubMed:32060358 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution8.0 - 13.0 Å
Structure data

0932

6lnt

EMDB-0932, PDB-6lnt:
Cryo-EM structure of immature Zika virus in complex with human antibody DV62.5 Fab
Method: EM (single particle) / Resolution: 8.0 Å

0933

6lnu

EMDB-0933, PDB-6lnu:
Cryo-EM structure of immature Zika virus
Method: EM (single particle) / Resolution: 9.0 Å

EMDB-0934:
Sub-tomogram averaging of immature Zika virus in complex with Fab DV62.5
Method: EM (subtomogram averaging) / Resolution: 13.0 Å

Source
  • Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013
  • homo sapiens (human)
  • zika virus (isolate zikv/human/french polynesia/10087pf/2013)
KeywordsVIRUS / immature Zika virus / human antibody / flavivirus

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