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TitleStructural and Functional Insights into Human Re-initiation Complexes.
Journal, issue, pagesMol Cell, Vol. 67, Issue 3, Page 447-456.e7, Year 2017
Publish dateAug 3, 2017
AuthorsMelanie Weisser / Tanja Schäfer / Marc Leibundgut / Daniel Böhringer / Christopher Herbert Stanley Aylett / Nenad Ban /
PubMed AbstractAfter having translated short upstream open reading frames, ribosomes can re-initiate translation on the same mRNA. This process, referred to as re-initiation, controls the translation of a large ...After having translated short upstream open reading frames, ribosomes can re-initiate translation on the same mRNA. This process, referred to as re-initiation, controls the translation of a large fraction of mammalian cellular mRNAs, many of which are important in cancer. Key ribosomal binding proteins involved in re-initiation are the eukaryotic translation initiation factor 2D (eIF2D) or the homologous complex of MCT-1/DENR. We determined the structures of these factors bound to the human 40S ribosomal subunit in complex with initiator tRNA positioned on an mRNA start codon in the P-site using a combination of cryoelectron microscopy and X-ray crystallography. The structures, supported by biochemical experiments, reveal how eIF2D emulates the function of several canonical translation initiation factors by using three independent, flexibly connected RNA binding domains to simultaneously monitor codon-anticodon interactions in the ribosomal P-site and position the initiator tRNA.
External linksMol Cell / PubMed:28732596
MethodsEM (single particle) / X-ray diffraction
Resolution1.8 - 4.2 Å
Structure data

3770

5oa3

EMDB-3770, PDB-5oa3:
Human 40S-eIF2D-re-initiation complex
Method: EM (single particle) / Resolution: 4.2 Å

PDB-5oa9:
Human translation re-initiation complex containing eIF2D
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • hepatitis c virus
  • saccharomyces cerevisiae (brewer's yeast)
  • Human (human)
KeywordsTRANSLATION / translation re-initiation complex / small ribosomal subunit / RNA binding protein / eukaryotic translation initiation factor / translation initiation factor

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