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TitleArchitecture of a transcribing-translating expressome.
Journal, issue, pagesScience, Vol. 356, Issue 6334, Page 194-197, Year 2017
Publish dateApr 14, 2017
AuthorsR Kohler / R A Mooney / D J Mills / R Landick / P Cramer /
PubMed AbstractDNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can ...DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.
External linksScience / PubMed:28408604 / PubMed Central
MethodsEM (single particle)
Resolution6.4 - 7.6 Å
Structure data

EMDB-3579:
30S ribosomal subunit with partial density for ribosomal protein S1
Method: EM (single particle) / Resolution: 6.4 Å

3580

5my1

EMDB-3580, PDB-5my1:
E. coli expressome
Method: EM (single particle) / Resolution: 7.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • Escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)
KeywordsRIBOSOME / expressome ribosome RNA polymerase

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