Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin.
Journal, issue, pages	Elife, Vol. 6, Year 2017
Publish date	Mar 21, 2017
Authors	Katharina van Pee / Alexander Neuhaus / Edoardo D'Imprima / Deryck J Mills / Werner Kühlbrandt / Özkan Yildiz /
PubMed Abstract	Many pathogenic bacteria produce pore-forming toxins to attack and kill human cells. We have determined the 4.5 Å structure of the ~2.2 MDa pore complex of pneumolysin, the main virulence factor of ...Many pathogenic bacteria produce pore-forming toxins to attack and kill human cells. We have determined the 4.5 Å structure of the ~2.2 MDa pore complex of pneumolysin, the main virulence factor of , by cryoEM. The pneumolysin pore is a 400 Å ring of 42 membrane-inserted monomers. Domain 3 of the soluble toxin refolds into two ~85 Å β-hairpins that traverse the lipid bilayer and assemble into a 168-strand β-barrel. The pore complex is stabilized by salt bridges between β-hairpins of adjacent subunits and an internal α-barrel. The apolar outer barrel surface with large sidechains is immersed in the lipid bilayer, while the inner barrel surface is highly charged. Comparison of the cryoEM pore complex to the prepore structure obtained by electron cryo-tomography and the x-ray structure of the soluble form reveals the detailed mechanisms by which the toxin monomers insert into the lipid bilayer to perforate the target membrane.
External links	Elife / PubMed:28323617 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.45 - 4.5 Å
Structure data	4118 5ly6 EMDB-4118, PDB-5ly6: CryoEM structure of the membrane pore complex of Pneumolysin at 4.5A Method: EM (single particle) / Resolution: 4.5 Å PDB-5aoe: Crystal structure of pneumolysin D168A mutant. Method: X-RAY DIFFRACTION / Resolution: 2.5 Å PDB-5aof: Crystal structure of pneumolysin deletion mutant Delta146_147. Method: X-RAY DIFFRACTION / Resolution: 2.45 Å
Chemicals	ChemComp-HOH: WATER / Water
Source	streptococcus pneumoniae (bacteria) streptococcus pneumoniae serotype 2 (strain d39 / nctc 7466) (bacteria)
Keywords	TOXIN / CHOLESTEROL DEPENDENT CYTOLYSIN / PORE FORMING TOXIN / Bacterial toxins / pore complex / cryoEM structure / cholesterol-dependent cytolysin / Pneumolysin / membrane pore