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Structure paper

TitleStructure of mammalian respiratory complex I.
Journal, issue, pagesNature, Vol. 536, Issue 7616, Page 354-358, Year 2016
Publish dateAug 18, 2016
AuthorsJiapeng Zhu / Kutti R Vinothkumar / Judy Hirst /
PubMed AbstractComplex I (NADH:ubiquinone oxidoreductase), one of the largest membrane-bound enzymes in the cell, powers ATP synthesis in mammalian mitochondria by using the reducing potential of NADH to drive ...Complex I (NADH:ubiquinone oxidoreductase), one of the largest membrane-bound enzymes in the cell, powers ATP synthesis in mammalian mitochondria by using the reducing potential of NADH to drive protons across the inner mitochondrial membrane. Mammalian complex I (ref. 1) contains 45 subunits, comprising 14 core subunits that house the catalytic machinery (and are conserved from bacteria to humans) and a mammalian-specific cohort of 31 supernumerary subunits. Knowledge of the structures and functions of the supernumerary subunits is fragmentary. Here we describe a 4.2-Å resolution single-particle electron cryomicroscopy structure of complex I from Bos taurus. We have located and modelled all 45 subunits, including the 31 supernumerary subunits, to provide the entire structure of the mammalian complex. Computational sorting of the particles identified different structural classes, related by subtle domain movements, which reveal conformationally dynamic regions and match biochemical descriptions of the 'active-to-de-active' enzyme transition that occurs during hypoxia. Our structures therefore provide a foundation for understanding complex I assembly and the effects of mutations that cause clinically relevant complex I dysfunctions, give insights into the structural and functional roles of the supernumerary subunits and reveal new information on the mechanism and regulation of catalysis.
External linksNature / PubMed:27509854 / PubMed Central
MethodsEM (single particle)
Resolution4.27 - 5.6 Å
Structure data

4032

5lc5

EMDB-4032, PDB-5lc5:
Structure of mammalian respiratory Complex I, class2
Method: EM (single particle) / Resolution: 4.35 Å

4040

5ldw

EMDB-4040, PDB-5ldw:
Structure of mammalian respiratory Complex I, class1
Method: EM (single particle) / Resolution: 4.27 Å

4041

5ldx

EMDB-4041, PDB-5ldx:
Structure of mammalian respiratory Complex I, class3.
Method: EM (single particle) / Resolution: 5.6 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

ChemComp-ZN:
Unknown entry

Source
  • bos taurus (cattle)
  • Bovine (cattle)
KeywordsOXIDOREDUCTASE / NADH:ubiquinone oxidoreductase / multienzyme complexes / Complex I / mitochondria

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