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TitleNear-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.
Journal, issue, pagesJ Mol Biol, Vol. 429, Issue 1, Page 79-87, Year 2017
Publish dateJan 6, 2017
AuthorsXiaowu Li / Niyun Zhou / Wenyuan Chen / Bin Zhu / Xurong Wang / Bin Xu / Jiawei Wang / Hongrong Liu / Lingpeng Cheng /
PubMed AbstractSingle-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than ...Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5Å) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-Å diameter at 3.3-Å resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-Å resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-Å resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.
External linksJ Mol Biol / PubMed:27914893
MethodsEM (single particle)
Resolution3.3 - 3.9 Å
Structure data

9564

5h0r

EMDB-9564, PDB-5h0r:
RNA dependent RNA polymerase ,vp4,dsRNA
Method: EM (single particle) / Resolution: 3.9 Å

9565

5h0s

EMDB-9565, PDB-5h0s:
EM Structure of VP1A and VP1B
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • bombyx mori cytoplasmic polyhedrosis virus
  • dendrolimus punctatus cypovirus 1
  • cypovirus (cytoplasmic polyhedrosis viruses)
  • bombyx mori cypovirus 1
KeywordsTRANSFERASE/RNA / structural classification / TRANSFERASE-RNA complex / TRANSFERASE

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