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TitleA Molecular-Level Account of the Antigenic Hantaviral Surface.
Journal, issue, pagesCell Rep, Vol. 15, Issue 5, Page 959-967, Year 2016
Publish dateMay 3, 2016
AuthorsSai Li / Ilona Rissanen / Antra Zeltina / Jussi Hepojoki / Jayna Raghwani / Karl Harlos / Oliver G Pybus / Juha T Huiskonen / Thomas A Bowden /
PubMed AbstractHantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high- ...Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.
External linksCell Rep / PubMed:27117403 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction / EM (tomography)
Resolution2.28 - 15.6 Å
Structure data

EMDB-3364, PDB-5fyn:
Sub-tomogram averaging of Tula virus glycoprotein spike
Method: EM (subtomogram averaging) / Resolution: 15.6 Å

PDB-5fxu:
Crystal Structure of Puumala virus Gn glycoprotein ectodomain
Method: X-RAY DIFFRACTION / Resolution: 2.28 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • puumala orthohantavirus
  • puumala virus
KeywordsVIRAL PROTEIN / HANTAVIRUS / GN / VIRAL GLYCOPROTEIN / BUNYAVIRUS / PUUMALA VIRUS / GLYCOPROTEIN / TULA VIRUS / MEMBRANE PROTEIN / RECEPTOR BINDING

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