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TitleRibosome. The complete structure of the 55S mammalian mitochondrial ribosome.
Journal, issue, pagesScience, Vol. 348, Issue 6232, Page 303-308, Year 2015
Publish dateApr 17, 2015
AuthorsBasil J Greber / Philipp Bieri / Marc Leibundgut / Alexander Leitner / Ruedi Aebersold / Daniel Boehringer / Nenad Ban /
PubMed AbstractMammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of ...Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.
External linksScience / PubMed:25837512
MethodsEM (single particle)
Resolution3.6 - 3.8 Å
Structure data

2913

5aj3

EMDB-2913: Cryo-EM reconstruction of the mammalian 28S mitoribosomal subunit
PDB-5aj3: Structure of the small subunit of the mammalian mitoribosome
Method: EM (single particle) / Resolution: 3.6 Å

2914

5aj4

EMDB-2914: Cryo-EM reconstruction of the mammalian 55S mitoribosome
PDB-5aj4: Structure of the 55S mammalian mitoribosome.
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-HOH:
WATER / Water

Source
  • sus scrofa (pig)
KeywordsRIBOSOME / TRANSLATION / MITOCHONDRIA / MAMMALIAN 55S MITORIBOSOME / MAMMALIAN 55S MITOCHONDRIAL RIBOSOME / 28S SMALL SUBUNIT / MRNA / TRNA / DECODING CENTER / CRYO-EM / SINGLE PARTICLE ANALYSIS / 39S LARGE SUBUNIT / PTC / CRYO- EM

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